EC Number | Cloned (Comment) | Organism |
---|---|---|
5.1.3.8 | expression in Escherichia coli | Anabaena sp. CH1 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.1.3.8 | of its apoform | Anabaena sp. CH1 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.1.3.8 | C370A | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | E218A | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | E218D | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | E242Q | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | E308A | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | E308D | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | H239A | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | H239N | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | H372A | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | H372I | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | H372N | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | N179A | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | R375A | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | R375K | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | R57A | site directed mutagenesis | Anabaena sp. CH1 |
5.1.3.8 | R57K | site directed mutagenesis | Anabaena sp. CH1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.1.3.8 | 5.2 | - |
N-acetyl-D-glucosamine | E242Q, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 7.4 | - |
N-acetyl-D-glucosamine | R375K, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 9.2 | - |
N-acetyl-D-mannosamine | R375K, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 13.4 | - |
N-acetyl-D-glucosamine | E218D, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 13.9 | - |
N-acetyl-D-mannosamine | R375A, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 14.8 | - |
N-acetyl-D-mannosamine | wild type, at pH 7, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 15.5 | - |
N-acetyl-D-mannosamine | E218A, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 15.6 | - |
N-acetyl-D-mannosamine | wild type, at pH 8, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 16.7 | - |
N-acetyl-D-glucosamine | C370A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 16.7 | - |
N-acetyl-D-glucosamine | R375A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 21 | - |
N-acetyl-D-mannosamine | E218D, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 21.3 | - |
N-acetyl-D-mannosamine | C370A, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 24.4 | - |
N-acetyl-D-glucosamine | E218A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 25.4 | - |
N-acetyl-D-glucosamine | wild type, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 27.2 | - |
N-acetyl-D-mannosamine | N179A, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 32.7 | - |
N-acetyl-D-mannosamine | wild type, at pH 9, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 45.7 | - |
N-acetyl-D-glucosamine | N179A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 53.8 | - |
N-acetyl-D-mannosamine | E242Q, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
5.1.3.8 | 57.33 | - |
N-acetyl-D-mannosamine | wild type, at pH 6 in the presence of 1 mM ATP | Anabaena sp. CH1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.1.3.8 | Anabaena sp. CH1 | A4UA16 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.1.3.8 | of the recombinant proteins, wild type and mutants by immobilized nickel-ion chromatography | Anabaena sp. CH1 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
5.1.3.8 | 5.6 | - |
E242Q, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 6.2 | - |
R375A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 6.6 | - |
E218A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 8.1 | - |
E242Q, in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 8.9 | - |
R375K, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 22.8 | - |
E218A, in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 46.5 | - |
E218D, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 50.8 | - |
N179A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 54.8 | - |
R375A, in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 85.9 | - |
C370A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 94.3 | - |
R375K, in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 123.8 | - |
wild type, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 256.4 | - |
E218D, in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 258.5 | - |
N179A, in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 351 | - |
C370A, in the presence of 1 mM ATP | Anabaena sp. CH1 |
5.1.3.8 | 525.8 | - |
wild type, in the presence of 1 mM ATP | Anabaena sp. CH1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.3.8 | N-acetyl-D-mannosamine | - |
Anabaena sp. CH1 | N-acetyl-D-glucosamine | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.1.3.8 | bGlcNAc 2-epimerase | recombinant protein | Anabaena sp. CH1 |
5.1.3.8 | GlcNAc 2-epimerase | - |
Anabaena sp. CH1 |
5.1.3.8 | N-acyl-D-glucosamine 2-epimerase | - |
Anabaena sp. CH1 |
5.1.3.8 | N-acylglucosamine 2-epimerase | - |
Anabaena sp. CH1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.8 | 7 | - |
- |
Anabaena sp. CH1 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
5.1.3.8 | 7 | 9.5 | - |
Anabaena sp. CH1 |