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Literature summary extracted from

  • Lee, Y.C.; Wu, H.M.; Chang, Y.N.; Wang, W.C.; Hsu, W.H.
    The central cavity from the (alpha/alpha)6 barrel structure of Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase contains two key histidine residues for reversible conversion (2007), J. Mol. Biol., 367, 895-908.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.8 expression in Escherichia coli Anabaena sp. CH1

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.3.8 of its apoform Anabaena sp. CH1

Protein Variants

EC Number Protein Variants Comment Organism
5.1.3.8 C370A site directed mutagenesis Anabaena sp. CH1
5.1.3.8 E218A site directed mutagenesis Anabaena sp. CH1
5.1.3.8 E218D site directed mutagenesis Anabaena sp. CH1
5.1.3.8 E242Q site directed mutagenesis Anabaena sp. CH1
5.1.3.8 E308A site directed mutagenesis Anabaena sp. CH1
5.1.3.8 E308D site directed mutagenesis Anabaena sp. CH1
5.1.3.8 H239A site directed mutagenesis Anabaena sp. CH1
5.1.3.8 H239N site directed mutagenesis Anabaena sp. CH1
5.1.3.8 H372A site directed mutagenesis Anabaena sp. CH1
5.1.3.8 H372I site directed mutagenesis Anabaena sp. CH1
5.1.3.8 H372N site directed mutagenesis Anabaena sp. CH1
5.1.3.8 N179A site directed mutagenesis Anabaena sp. CH1
5.1.3.8 R375A site directed mutagenesis Anabaena sp. CH1
5.1.3.8 R375K site directed mutagenesis Anabaena sp. CH1
5.1.3.8 R57A site directed mutagenesis Anabaena sp. CH1
5.1.3.8 R57K site directed mutagenesis Anabaena sp. CH1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.3.8 5.2
-
N-acetyl-D-glucosamine E242Q, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 7.4
-
N-acetyl-D-glucosamine R375K, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 9.2
-
N-acetyl-D-mannosamine R375K, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 13.4
-
N-acetyl-D-glucosamine E218D, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 13.9
-
N-acetyl-D-mannosamine R375A, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 14.8
-
N-acetyl-D-mannosamine wild type, at pH 7, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 15.5
-
N-acetyl-D-mannosamine E218A, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 15.6
-
N-acetyl-D-mannosamine wild type, at pH 8, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 16.7
-
N-acetyl-D-glucosamine C370A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 16.7
-
N-acetyl-D-glucosamine R375A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 21
-
N-acetyl-D-mannosamine E218D, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 21.3
-
N-acetyl-D-mannosamine C370A, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 24.4
-
N-acetyl-D-glucosamine E218A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 25.4
-
N-acetyl-D-glucosamine wild type, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 27.2
-
N-acetyl-D-mannosamine N179A, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 32.7
-
N-acetyl-D-mannosamine wild type, at pH 9, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 45.7
-
N-acetyl-D-glucosamine N179A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 53.8
-
N-acetyl-D-mannosamine E242Q, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 57.33
-
N-acetyl-D-mannosamine wild type, at pH 6 in the presence of 1 mM ATP Anabaena sp. CH1

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.8 Anabaena sp. CH1 A4UA16
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.8 of the recombinant proteins, wild type and mutants by immobilized nickel-ion chromatography Anabaena sp. CH1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.3.8 5.6
-
E242Q, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 6.2
-
R375A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 6.6
-
E218A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 8.1
-
E242Q, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 8.9
-
R375K, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 22.8
-
E218A, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 46.5
-
E218D, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 50.8
-
N179A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 54.8
-
R375A, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 85.9
-
C370A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 94.3
-
R375K, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 123.8
-
wild type, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 256.4
-
E218D, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 258.5
-
N179A, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 351
-
C370A, in the presence of 1 mM ATP Anabaena sp. CH1
5.1.3.8 525.8
-
wild type, in the presence of 1 mM ATP Anabaena sp. CH1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.8 N-acetyl-D-mannosamine
-
Anabaena sp. CH1 N-acetyl-D-glucosamine
-
r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.8 bGlcNAc 2-epimerase recombinant protein Anabaena sp. CH1
5.1.3.8 GlcNAc 2-epimerase
-
Anabaena sp. CH1
5.1.3.8 N-acyl-D-glucosamine 2-epimerase
-
Anabaena sp. CH1
5.1.3.8 N-acylglucosamine 2-epimerase
-
Anabaena sp. CH1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.3.8 7
-
-
Anabaena sp. CH1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.3.8 7 9.5
-
Anabaena sp. CH1