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Literature summary extracted from

  • Sekulic, N.; Dietrich, K.; Paarmann, I.; Ort, S.; Konrad, M.; Lavie, A.
    Elucidation of the active conformation of the APS-kinase domain of human PAPS synthetase 1 (2007), J. Mol. Biol., 367, 488-500.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 the APS-kinase domain (25-227 amino acid resiudes) of the PAPSS1 coding region are expressed as a GST-fusion protein in Escherichia coli BL-21 Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.25 crystal structures of the PAPSS1 APS-kinase domain in complex with APS and in complex with the products PAPS and ADP are solved, both structures of isolated domain, obtained in different crystal forms, reveal symmetrical dimers Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.25 EDTA assay with 0.25 mM EDTA Homo sapiens
2.7.1.25 K+ assay with 100 mM KCl Homo sapiens
2.7.1.25 Mg2+ assay with 2.5 mM MgCl2 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 protein solution is loaded on a Glutathione-Sepharose Fast Flow column and further purified by gel-filtration chromatography using Superdex 200 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 MgATP2- + adenylyl sulfate
-
 Homo sapiens MgADP- + 3'-phosphoadenylyl sulfate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.1.25 3'-phosphoadenosine 5'-phosphosulfate synthetase1
-
 Homo sapiens
2.7.1.25 PAPSS1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.25 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.25 7.5
-
 assay at Homo sapiens