EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.23 | additional information | the enzyme is specifically induced by 3-hydroxybenzoate, no activation by 4-hydroxybenzoate | Comamonas testosteroni |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.23 | gene mobA, promoter study, primer extension method, transription regulation/repression by MobR, genetic organization, overview, overexpression of mobA and of a mobA-mobR fusion construct in Escherichia coli | Comamonas testosteroni |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.23 | MobR | a 42 kDa dimeric transcriptional regulator of the MarR family, encoded by an open reading frame mobR in the upstream region of mobA, binds to the target DNA and negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate, binding kinetics, overview | Comamonas testosteroni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.23 | 3-hydroxybenzoate + NADH + O2 | Comamonas testosteroni | initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.13.23 | 3-hydroxybenzoate + NADH + O2 | Comamonas testosteroni KH122-3s | initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.23 | Comamonas testosteroni | - |
- |
- |
1.14.13.23 | Comamonas testosteroni KH122-3s | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.23 | additional information | the aerobic soil bacterium that utilizes 3-hydroxybenzoate as a sole carbon and energy source | Comamonas testosteroni | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.23 | 0.002 | - |
uninduced wild-type strain | Comamonas testosteroni |
1.14.13.23 | 0.055 | - |
wild-type strain induced by 3-hydroxybenzoate | Comamonas testosteroni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.23 | 3-hydroxybenzoate + NADH + O2 | - |
Comamonas testosteroni | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.13.23 | 3-hydroxybenzoate + NADH + O2 | initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate | Comamonas testosteroni | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.13.23 | 3-hydroxybenzoate + NADH + O2 | - |
Comamonas testosteroni KH122-3s | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? | |
1.14.13.23 | 3-hydroxybenzoate + NADH + O2 | initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate | Comamonas testosteroni KH122-3s | 3,4-dihydroxybenzoate + NAD+ + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.23 | 3-hydroxybenzoate hydroxylase | - |
Comamonas testosteroni |
1.14.13.23 | MobA | - |
Comamonas testosteroni |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.23 | 24 | - |
assay at | Comamonas testosteroni |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.23 | FAD | - |
Comamonas testosteroni | |
1.14.13.23 | NADPH | - |
Comamonas testosteroni |