Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hiromoto, T.; Matsue, H.; Yoshida, M.; Tanaka, T.; Higashibata, H.; Hosokawa, K.; Yamaguchi, H.; Fujiwara, S.
    Characterization of MobR, the 3-hydroxybenzoate-responsive transcriptional regulator for the 3-hydroxybenzoate hydroxylase gene of Comamonas testosteroni KH122-3s (2006), J. Mol. Biol., 364, 863-877.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.23 additional information the enzyme is specifically induced by 3-hydroxybenzoate, no activation by 4-hydroxybenzoate Comamonas testosteroni

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.23 gene mobA, promoter study, primer extension method, transription regulation/repression by MobR, genetic organization, overview, overexpression of mobA and of a mobA-mobR fusion construct in Escherichia coli Comamonas testosteroni

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.23 MobR a 42 kDa dimeric transcriptional regulator of the MarR family, encoded by an open reading frame mobR in the upstream region of mobA, binds to the target DNA and negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate, binding kinetics, overview Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.23 3-hydroxybenzoate + NADH + O2 Comamonas testosteroni initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate 3,4-dihydroxybenzoate + NAD+ + H2O
-
?
1.14.13.23 3-hydroxybenzoate + NADH + O2 Comamonas testosteroni KH122-3s initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate 3,4-dihydroxybenzoate + NAD+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.23 Comamonas testosteroni
-
-
-
1.14.13.23 Comamonas testosteroni KH122-3s
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.23 additional information the aerobic soil bacterium that utilizes 3-hydroxybenzoate as a sole carbon and energy source Comamonas testosteroni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.23 0.002
-
uninduced wild-type strain Comamonas testosteroni
1.14.13.23 0.055
-
wild-type strain induced by 3-hydroxybenzoate Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.23 3-hydroxybenzoate + NADH + O2
-
Comamonas testosteroni 3,4-dihydroxybenzoate + NAD+ + H2O
-
?
1.14.13.23 3-hydroxybenzoate + NADH + O2 initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate Comamonas testosteroni 3,4-dihydroxybenzoate + NAD+ + H2O
-
?
1.14.13.23 3-hydroxybenzoate + NADH + O2
-
Comamonas testosteroni KH122-3s 3,4-dihydroxybenzoate + NAD+ + H2O
-
?
1.14.13.23 3-hydroxybenzoate + NADH + O2 initial step of the degradation in the meta-cleavage pathway leading to tricarboxylic acid cycle intermediates, MobR negatively regulates the expression of mobA, the repression is relieved by binding of 3-hydroxybenzoate Comamonas testosteroni KH122-3s 3,4-dihydroxybenzoate + NAD+ + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.23 3-hydroxybenzoate hydroxylase
-
Comamonas testosteroni
1.14.13.23 MobA
-
Comamonas testosteroni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.23 24
-
assay at Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.23 FAD
-
Comamonas testosteroni
1.14.13.23 NADPH
-
Comamonas testosteroni