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Literature summary extracted from

  • Lesnyak, D.V.; Sergiev, P.V.; Bogdanov, A.A.; Dontsova, O.A.
    Identification of Escherichia coli m2G methyltransferases: I. the ycbY gene encodes a methyltransferase specific for G2445 of the 23 S rRNA (2006), J. Mol. Biol., 364, 20-25.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.173 YcbY protein carrying a C-terminal His6 tag is expressed in Escherichia coli Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.173 79000
-
 x * 79000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.173 S-adenosyl-L-methionine + guanine2445 in 23S rRNA Escherichia coli N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.173 Escherichia coli P75864
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.173 recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.173 S-adenosyl-L-methionine + guanine2445 in 23S rRNA N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?
2.1.1.173 S-adenosyl-L-methionine + guanine2445 in 23S rRNA recombinant YcbY protein is able to methylate 23S rRNA purified from the ycbY knock-out strain in vitro, assembled 50S subunits are not a substrate for the methylase Escherichia coli S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.173 ? x * 79000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.173 RlmL
-
 Escherichia coli
2.1.1.173 ycbY
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.173 malfunction knock-out of the ycbY gene leads to loss of modification at G2445 and growth retardation. Growth competition with the parental wild-type strain leads to a gradual decrease in the knock-out strain cells proportion in the media Escherichia coli
2.1.1.173 physiological function it is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center Escherichia coli