Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Malito, E.; Sekulic, N.; Too, W.C.; Konrad, M.; Lavie, A.
    Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine (2006), J. Mol. Biol., 364, 136-151.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 expressed in Escherichia coli strain BL21(DE3) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.32 sitting drop vapour diffusion using 25 mM Tris/HCl (pH 7.5), 25 mM KCl, 0.5 mM dithiothreitol, 0.0005 ml of 0.1 M MgCl2 in 12-20% PEG 3350 with 0.2 M NaF Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 hemicholinium
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 0.07
-
choline in 100 mM Tris-HCl buffer (pH 7.5) containing 100 mM KCl, 10 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.25 mM NADH, 4 units pyruvate kinase, and 5 units lactate dehydrogenase Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.32 52000
-
estimated from amino acid sequence Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Homo sapiens P35790
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32 gluthatione-Sepharose column chromatography, Resource Q column chromatography and Superdex-200 gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.32 liver
-
Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
Homo sapiens ADP + O-phosphocholine
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.32 dimer x-ray crystallography Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 choline kinase isoform alpha2
-
Homo sapiens
2.7.1.32 CKalpha2
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.32 71.5
-
choline in 100 mM Tris-HCl buffer (pH 7.5) containing 100 mM KCl, 10 mM MgCl2, 0.5 mM phosphoenolpyruvate, 0.25 mM NADH, 4 units pyruvate kinase, and 5 units lactate dehydrogenase Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.32 ATP
-
Homo sapiens