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Literature summary extracted from

  • Newman, J.A.; Das, S.K.; Sedelnikova, S.E.; Rice, D.W.
    The crystal structure of an ADP complex of Bacillus subtilis pyridoxal kinase provides evidence for the parallel emergence of enzyme activity during evolution (2006), J. Mol. Biol., 363, 520-530.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.35 expressed in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.35 with 28% (w/v) PEG 4000, 0.17 M sodium acetate trihydrate, 0.1 M Tris-HCl (pH 8.5) as the precipitant in the presence of 10 mM ADP and 10 mM MgCl Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.35 Mg2+ active in the presence of Mg2+ Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.35 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.35 Hi-Load Superdex 200 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.35 ATP + pyridoxal
-
 Bacillus subtilis ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxamine
-
 Bacillus subtilis ADP + pyridoxamine 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxine
-
 Bacillus subtilis ADP + pyridoxine 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.35 dimer x-ray crystallography Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.35 PdxK
-
 Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.35 ATP
-
 Bacillus subtilis