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Literature summary extracted from

  • Zhang, N.; Liu, L.; Liu, F.; Wagner, C.R.; Hanna, P.E.; Walters, K.J.
    NMR-based model reveals the structural determinants of mammalian arylamine N-acetyltransferase substrate specificity (2006), J. Mol. Biol., 363, 188-200.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 0.049
-
 4-Aminobenzoic acid pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 0.061
-
 4-Aminobenzoic acid pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Mesocricetus auratus
2.3.1.5 0.17
-
 4-amino-3-methylbenzoic acid pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 0.18
-
 4-amino-3-methylbenzoic acid pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Mesocricetus auratus
2.3.1.5 1.1
-
 2-toluidine pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Mesocricetus auratus
2.3.1.5 1.2
-
 2-toluidine pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 6.6
-
 2-toluidine pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 11
-
 4-amino-3-methylbenzoic acid pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 166
-
 4-Aminobenzoic acid pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid Mesocricetus auratus
-
 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid Homo sapiens
-
 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid Mesocricetus auratus
-
 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid Homo sapiens a sunscreen additive 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobiphenyl Mesocricetus auratus
-
 4-nitrophenol + N-acetyl-4-aminobiphenyl
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobiphenyl Homo sapiens a tobacco smoke carcinogen 4-nitrophenol + N-acetyl-4-aminobiphenyl
-
 ?
2.3.1.5 additional information Mesocricetus auratus the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens ?
-
 ?
2.3.1.5 additional information Homo sapiens the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Homo sapiens P11245 isozyme NAT2; isozyme NAT2
-
2.3.1.5 Homo sapiens P18440 isozyme NAT1; isozyme NAT1
-
2.3.1.5 Mesocricetus auratus P50293 isozyme NAT2; isozyme NAT2
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 additional information
-
-
 Mesocricetus auratus
2.3.1.5 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid
-
 Mesocricetus auratus 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid
-
 Homo sapiens 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid substrate of isozyme NAT1 Homo sapiens 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-amino-3-methylbenzoic acid very low activity with isozyme NAT2 Homo sapiens 4-nitrophenol + N-acetyl-4-amino-3-methylbenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid
-
 Mesocricetus auratus 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid a sunscreen additive Homo sapiens 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid substrate of isozyme NAT1 Homo sapiens 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid very low activity with isozyme NAT2 Homo sapiens 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobiphenyl
-
 Mesocricetus auratus 4-nitrophenol + N-acetyl-4-aminobiphenyl
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobiphenyl a tobacco smoke carcinogen Homo sapiens 4-nitrophenol + N-acetyl-4-aminobiphenyl
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobiphenyl substrate of isozyme NAT1 Homo sapiens 4-nitrophenol + N-acetyl-4-aminobiphenyl
-
 ?
2.3.1.5 4-nitrophenyl acetate + 4-aminobiphenyl very low activity with isozyme NAT2 Homo sapiens 4-nitrophenol + N-acetyl-4-aminobiphenyl
-
 ?
2.3.1.5 acetyl-CoA + 2-toluidine
-
 Mesocricetus auratus CoA + N-acetyl-2-toluidine
-
 ?
2.3.1.5 acetyl-CoA + 2-toluidine
-
 Homo sapiens CoA + N-acetyl-2-toluidine
-
 ?
2.3.1.5 additional information the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens Mesocricetus auratus ?
-
 ?
2.3.1.5 additional information the enzyme catalyzes the acetylation of arylamines, a key step in the detoxification of many carcinogens Homo sapiens ?
-
 ?
2.3.1.5 additional information poor activity wit 2-toluidine and other arylamines linked to bladder cancer Mesocricetus auratus ?
-
 ?
2.3.1.5 additional information poor activity wit 2-toluidine and other arylamines linked to bladder cancer Homo sapiens ?
-
 ?
2.3.1.5 additional information poor activity with 2-toluidine and other arylamines linked to bladder cancer Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.5 More secondary structure analysis of isozyme NAT1, comparison to the hamster isozyme NAT2, homology modeling, overview Homo sapiens
2.3.1.5 More secondary structure analysis of isozyme NAT2, comparison to the human isozyme NAT1, homology modeling, overview Mesocricetus auratus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 NAT
-
 Mesocricetus auratus
2.3.1.5 NAT
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 23
-
 assay at Mesocricetus auratus
2.3.1.5 23
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.5 1.3
-
 4-amino-3-methylbenzoic acid pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 4.8
-
 2-toluidine pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Mesocricetus auratus
2.3.1.5 6.3
-
 2-toluidine pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 14.2
-
 4-amino-3-methylbenzoic acid pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Mesocricetus auratus
2.3.1.5 16.6
-
 4-amino-3-methylbenzoic acid pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 21
-
 4-Aminobenzoic acid pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 72
-
 2-toluidine pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 127
-
 4-Aminobenzoic acid pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate Homo sapiens
2.3.1.5 230
-
 4-Aminobenzoic acid pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate Mesocricetus auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7
-
 assay at Mesocricetus auratus
2.3.1.5 7
-
 assay at Homo sapiens