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Literature summary extracted from

  • Liu, F.; Zhang, N.; Zhou, X.; Hanna, P.E.; Wagner, C.R.; Koepp, D.M.; Walters, K.J.
    Arylamine N-acetyltransferase aggregation and constitutive ubiquitylation (2006), J. Mol. Biol., 361, 482-492.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 expression of FLAG-tagged wild-type and mutant NAT2 in HEK-293T cells Mesocricetus auratus
2.3.1.5 expression of wild-type and mutant NAT1 in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.5 additional information humans harboring certain genetic variations within the NAT genes exhibit increased likelihood of developing various cancer types, especially urinary bladder cancer, polymorphisms, the mutants exhibit reduced cellular activity, which is proposed to be due to their constitutive ubiquitylation and enhanced proteasomal degradation, overview Homo sapiens
2.3.1.5 R64W naturally occuring polymorphism in gene NAT1, the R64W mutation also causes constitutive ubiquitinylation and NAT1 to aggregate in cultured cells, does not interfere with NAT catalysis in vitro, overall protein structure and thermostability are not compromised Homo sapiens
2.3.1.5 R64W naturally occuring polymorphism in gene NAT2, the R64W mutation also causes constitutive ubiquitinylation and NAT2 to aggregate in cultured cells, does not interfere with NAT catalysis in vitro, overall protein structure and thermostability are not compromised Mesocricetus auratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 0.23
-
 4-Aminobenzoic acid pH 7.0, 25°C, recombinant wild-type enzyme Mesocricetus auratus
2.3.1.5 0.37
-
 4-Aminobenzoic acid pH 7.0, 25°C, recombinant mutant R64W Mesocricetus auratus
2.3.1.5 4.81
-
 4-nitrophenyl acetate pH 7.0, 25°C, recombinant mutant R64W Mesocricetus auratus
2.3.1.5 10
-
 4-nitrophenyl acetate pH 7.0, 25°C, recombinant wild-type enzyme Mesocricetus auratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.5 additional information Mesocricetus auratus isozyme NAT1 acetylates and detoxifies arylamine carcinogens ?
-
 ?
2.3.1.5 additional information Homo sapiens isozyme NAT1 acetylates and detoxifies arylamine carcinogens ?
-
 ?
2.3.1.5 additional information Mesocricetus auratus isozyme NAT2 acetylates and detoxifies arylamine carcinogens ?
-
 ?
2.3.1.5 additional information Homo sapiens isozyme NAT2 acetylates and detoxifies arylamine carcinogens ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Homo sapiens P11245 isozyme NAT2; isozyme NAT2
-
2.3.1.5 Homo sapiens P18440 isozyme NAT1; isozyme NAT1
-
2.3.1.5 Mesocricetus auratus P50292 isozyme NAT1; isozyme NAT1
-
2.3.1.5 Mesocricetus auratus P50293 isozyme NAT2; isozyme NAT2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.5 recombinant wild-type and mutant enzymes NAT2 from HEK-293T cells by affinity chromatography Mesocricetus auratus
2.3.1.5 recombinant wild-type and mutant NAT1 from Escherichia coli Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 additional information
-
-
 Mesocricetus auratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 4-nitrophenyl acetate + 4-aminobenzoic acid
-
 Mesocricetus auratus 4-nitrophenol + N-acetyl-4-aminobenzoic acid
-
 ?
2.3.1.5 additional information isozyme NAT1 acetylates and detoxifies arylamine carcinogens Mesocricetus auratus ?
-
 ?
2.3.1.5 additional information isozyme NAT1 acetylates and detoxifies arylamine carcinogens Homo sapiens ?
-
 ?
2.3.1.5 additional information isozyme NAT2 acetylates and detoxifies arylamine carcinogens Mesocricetus auratus ?
-
 ?
2.3.1.5 additional information isozyme NAT2 acetylates and detoxifies arylamine carcinogens Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 NAT
-
 Mesocricetus auratus
2.3.1.5 NAT
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.5 25
-
 assay at Mesocricetus auratus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.5 56.3
-
 half-life of the acetylated state of recombinant R64W NAT at 25 °C, pH 7.0, cooperative unfolding is lost for the hamster NAT2 R64W variant Mesocricetus auratus
2.3.1.5 56.3
-
 half-life of the acetylated state of recombinant R64W NAT2 at 25 °C, pH 7.0, cooperative unfolding is lost for the hamster NAT2 R64W variant Mesocricetus auratus
2.3.1.5 88.3
-
 half-life of the acetylated state of recombinant wild-type NAT at 25 °C, pH 7.0, temperature-dependent unfolding of the wild-type enzyme is highly cooperative Mesocricetus auratus
2.3.1.5 88.3
-
 half-life of the acetylated state of recombinant wild-type NAT2 at 25 °C, pH 7.0, temperature-dependent unfolding of the wild-type enzyme is highly cooperative Mesocricetus auratus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.5 265
-
 4-Aminobenzoic acid pH 7.0, 25°C, recombinant mutant R64W, with 4-nitrophenyl acetate Mesocricetus auratus
2.3.1.5 265
-
 4-Aminobenzoic acid pH 7.0, 25°C, recombinant wild-type enzyme, with 4-nitrophenyl acetate Mesocricetus auratus
2.3.1.5 620
-
 4-nitrophenyl acetate pH 7.0, 25°C, recombinant mutant R64W, with 4-aminobenzoic acid Mesocricetus auratus
2.3.1.5 620
-
 4-nitrophenyl acetate pH 7.0, 25°C, recombinant wild-type enzyme, with 4-aminobenzoic acid Mesocricetus auratus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.5 7
-
 assay at Mesocricetus auratus