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Literature summary extracted from

  • Oliveira, J.S.; Pereira, J.H.; Canduri, F.; Rodrigues, N.C.; de Souza, O.N.; de Azevedo, W.F.; Basso, L.A.; Santos, D.S.
    Crystallographic and pre-steady-state kinetics studies on binding of NADH to wild-type and isoniazid-resistant enoyl-ACP(CoA) reductase enzymes from Mycobacterium tuberculosis (2006), J. Mol. Biol., 359, 646-666.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.9 medicine tuberculosis, TB, target of anti-tubercular agents Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9
-
 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.1.9 wild-type enzyme, S94A, I47T and I21V mutants in complex with NADH at resolutions of 2.3 A, 2.2 A, 2.0 A, 1.9 A, respectively Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.9 I21V mutant, studied for understanding of the isoniazid drug resistance mechanism Mycobacterium tuberculosis
1.3.1.9 I47T mutant, studied for understanding of the isoniazid drug resistance mechanism Mycobacterium tuberculosis
1.3.1.9 S94A mutant, studied for understanding of the isoniazid drug resistance mechanism Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 isoniazid
-
 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.9 104900
-
 mutant I21V, estimated by gel-filtration chromatography Mycobacterium tuberculosis
1.3.1.9 108700
-
 mutant S94A, estimated by gel-filtration chromatography Mycobacterium tuberculosis
1.3.1.9 115900
-
 wild-type enzyme, estimated by gel-filtration chromatography Mycobacterium tuberculosis
1.3.1.9 118100
-
 mutant I47T, estimated by gel-filtration chromatography Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.9 acyl-[acyl-carrier protein] + NAD+ Mycobacterium tuberculosis
-
 trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 acyl-[acyl-carrier protein] + NAD+
-
 Mycobacterium tuberculosis trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.9 homotetramer
-
 Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 2-trans enoyl-acyl carrier protein reductase
-
 Mycobacterium tuberculosis
1.3.1.9 enoyl-ACP(CoA) reductase
-
 Mycobacterium tuberculosis
1.3.1.9 enoyl-[acyl-carrier-protein] reductase
-
 Mycobacterium tuberculosis
1.3.1.9 InhA
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NADH
-
 Mycobacterium tuberculosis