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Literature summary extracted from

  • Ramakrishnan, B.; Ramasamy, V.; Qasba, P.K.
    Structural snapshots of beta-1,4-galactosyltransferase-I along the kinetic pathway (2006), J. Mol. Biol., 357, 1619-1633.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.38 expression of mutants W312C/P401C and W312C/P401C/M340H enzymes in Escherichia coli, the wild-type enzyme forms inclusion bodies after expression in Escherichia coli, overview Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.38 purified recombinant M340H-Gal-T1 mutant in the open conformation as apo-enzyme, and its Mn2+ and Mn2+-UDP-Gal-bound complexes, hanging-drop vapor diffusion method using a protein solution at 10 mg/ml concentration with a precipitant containing sodium citratebuffer, pH 5.5, and 10% w/v PEG-4000, the crystals of the Mn2+ complex are grown in the droplet containing 10 mM MnCl2, while the crystals containing UDP-Gal and Mn2+ are grown in the presence of 17 mM each of MnCl2 and UDPย–Gal, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution Homo sapiens
2.4.1.38 purified recombinant pentenary complex of bovine M344H-Gal-T1 mutant-Mn2+-UDP-GalNAc-Glc-alpha-lactalbumin, hanging-drop vapor diffusion method, using 20 mg/ml of beta-M344H-Gal-T1 and 10 mg/ml of mouse alpha-lactalbumin in the presence of 10 mM each ofUDP-Gal and CaCl2, with the precipitant containing 100 mM NaCl, 100 mM Mes-NaOH buffer, pH 6.0, and 12.5% PEG 4000, the crystals of the complex cannot be obtained in the absence of CaCl2, X-ray diffraction structure determination and analysis at 1.9-2.0 A resolution Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.38 M344H site-directed mutagenesis, substrate binding structure in comparison to the wild-type enzyme, overview Bos taurus
2.4.1.38 W312C/P401C site-directed mutagenesis, the mutations facilitate invitro folding of the recombinantly expressed enzyme Homo sapiens
2.4.1.38 W312C/P401C/M340H site-directed mutagenesis, substrate binding structure in comparison to the wild-type enzyme, overview Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.38 Ca2+
-
Bos taurus
2.4.1.38 Mn2+ required, binds first in the catalytic cycle Bos taurus
2.4.1.38 Mn2+ required, binds first in the catalytic cycle, Met340 is involved Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.38 UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide Homo sapiens
-
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
?
2.4.1.38 UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide Bos taurus
-
UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Bos taurus
-
-
-
2.4.1.38 Homo sapiens
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.38 UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide reaction mechanism and structural changes Homo sapiens
2.4.1.38 UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide reaction mechanism and structural changes Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.38 UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
-
Homo sapiens UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
?
2.4.1.38 UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide
-
Bos taurus UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
?
2.4.1.38 UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide catalytic cycle: Mn2+ binds first before UDP-Gal, two flexible loops, a long and a short loop, change their conformation from open to closed Homo sapiens UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
?
2.4.1.38 UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide catalytic cycle: Mn2+ binds first before UDP-Gal, two flexible loops, a long and a short loop, change their conformation from open to closed Bos taurus UDP + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminylglycopeptide
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.38 More structure analysis, in vivo and invitro folding analysis, open and closed conformation change of the enzyme upon Mn2+ and substrate binding, overview Homo sapiens
2.4.1.38 More structure analysis, open and closed conformation change of the enzyme upon Mn2+ and substrate binding, overview Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.38 beta-1,4-galactosyltransferase-I
-
Homo sapiens
2.4.1.38 beta-1,4-galactosyltransferase-I
-
Bos taurus
2.4.1.38 Gal-T1
-
Homo sapiens
2.4.1.38 Gal-T1
-
Bos taurus