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Literature summary extracted from
- Isolation and characterization of vanadium bromoperoxidase from a marine macroalga, Ecklonia stolonifera (1998), J. Inorg. Biochem., 72, 23-28.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.18 | Cu2+ | - |
Ecklonia cava subsp. stolonifera |  |
| 1.11.1.18 | Fe2+ | - |
Ecklonia cava subsp. stolonifera | |
| 1.11.1.18 | Fe3+ | - |
Ecklonia cava subsp. stolonifera | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.18 | 0.00012 | - |
monochlorodimedone | pH 6, 25°C | Ecklonia cava subsp. stolonifera | |
| 1.11.1.18 | 0.001 | - |
Br- | pH 6, 25°C | Ecklonia cava subsp. stolonifera | |
| 1.11.1.18 | 0.052 | - |
H2O2 | pH 6, 25°C | Ecklonia cava subsp. stolonifera | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.18 | Vanadium | requires vanadium for enzyme activity. The enzyme activity increases ca. 250% with the action of V5+ on the isolated enzyme, since more than 2/3 of the protein molecules are in the apo form. This effect of V5+ addition is inhibited in phosphate buffer, probably because phosphate and vanadate compete for the active site | Ecklonia cava subsp. stolonifera | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | 83000 | - |
x * 83000, SDS-PAGE | Ecklonia cava subsp. stolonifera |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.11.1.18 | 1-propanol | completely intact in the presence of 50% methanol | Ecklonia cava subsp. stolonifera |
| 1.11.1.18 | Ethanol | completely intact in the presence of 50% methanol | Ecklonia cava subsp. stolonifera |
| 1.11.1.18 | Methanol | completely intact in the presence of 50% methanol | Ecklonia cava subsp. stolonifera |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.18 | Ecklonia cava subsp. stolonifera | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.18 | - |
Ecklonia cava subsp. stolonifera |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | 32.5 | - |
- |
Ecklonia cava subsp. stolonifera |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.18 | Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione | i.e. monochlorodimedone | Ecklonia cava subsp. stolonifera | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.18 | ? | x * 83000, SDS-PAGE | Ecklonia cava subsp. stolonifera |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.18 | vanadium bromoperoxidase | - |
Ecklonia cava subsp. stolonifera |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | 68 | - |
Tm-value | Ecklonia cava subsp. stolonifera |
| 1.11.1.18 | 80 | - |
10 min, complete loss of activity | Ecklonia cava subsp. stolonifera |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.18 | 6.5 | - |
- |
Ecklonia cava subsp. stolonifera |
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