Any feedback?
Literature summary extracted from
- Influence of cobalt substitution on the activity of iron-type nitrile hydratase: are cobalt type nitrile hydratases regulated by carbon monoxide? (2007), J. Inorg. Biochem., 101, 614-622.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.84 | nitrile hydratase from Comamonas testosteroni is expressed in Escherichia coli, strain TG1, overexpressed chaperonin system | Comamonas testosteroni |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.2.1.84 | additional information | Fe of an Fe-dependent recombinant nitrile hydratase (wild-type, NilFe) is replaced by Co generating an Co-substituted enzyme (NilCo) | Comamonas testosteroni |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.84 | 2-Nitrophenol | 10 microM, 40-50% residual activity of NilFe, 90% residual activity of NilCo | Comamonas testosteroni |  |
| 4.2.1.84 | 3-Nitrophenol | 10 microM, 40-50% residual activity of NilFe, fully active NilCo | Comamonas testosteroni | |
| 4.2.1.84 | 4-nitrophenol | 10 microM, 40-50% residual activity of NilFe, 80% residual activity of NilCo | Comamonas testosteroni | |
| 4.2.1.84 | 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | - |
Comamonas testosteroni | |
| 4.2.1.84 | acrylonitrile | - |
Comamonas testosteroni | |
| 4.2.1.84 | Butyric acid | - |
Comamonas testosteroni | |
| 4.2.1.84 | CO | inhibition of NilCo, reversible by photoactivation, no inhibition of NilFe | Comamonas testosteroni | |
| 4.2.1.84 | KCN | - |
Comamonas testosteroni | |
| 4.2.1.84 | NaNO2 | NilFe is unaffected by NaNO2 | Comamonas testosteroni | |
| 4.2.1.84 | NO | inhibition of NilCo and NilFe, reversible by photoactivation with 150 W white light | Comamonas testosteroni | |
| 4.2.1.84 | tertiary-butyl isonitrile | NilFe is unaffected by tertiary-butyl isonitrile | Comamonas testosteroni | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.84 | 0.18 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | +/- 0.04, substrate: 2-nitro-5-thiocyanato-benzoic acid, NilFe | Comamonas testosteroni | |
| 4.2.1.84 | 0.19 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | +/- 0.06, substrate: 2-nitro-5-thiocyanato-benzoic acid, NilCo | Comamonas testosteroni | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 22700 | - |
alpha2beta2, 2 * 22800 + 2 * 22700 | Comamonas testosteroni |
| 4.2.1.84 | 22800 | - |
alpha2beta2, 2 * 22800 + 2 * 22700 | Comamonas testosteroni |
| 4.2.1.84 | 90000 | - |
gel filtration | Comamonas testosteroni |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.84 | Comamonas testosteroni | - |
Fe-dependent, wild-type nitrile hydratase is compared with artificial, Co-dependent nitrile hydratase | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.84 | CoCl2, an overexpressed chaperonin system and butyric acid is necessary for fully soluble and active NilCo protein, 95% purity on 12% SDS-PAGE | Comamonas testosteroni |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | additional information | - |
vmax: 28 +/- 5 nmol/min/mg, NilCo | Comamonas testosteroni |
| 4.2.1.84 | additional information | - |
vmax: 30 +/- 5 nmol/min/mg, NilFe | Comamonas testosteroni |
| 4.2.1.84 | 0.018 | - |
0.5 mM NaNO2, NilFe | Comamonas testosteroni |
| 4.2.1.84 | 15 | - |
+/- 5, substrate: 2-nitro-5-thiocyanato-benzoic acid, NilCo | Comamonas testosteroni |
| 4.2.1.84 | 15 | - |
+/- 5, substrate: 2-nitro-5-thiocyanato-benzoic acid, NilFe | Comamonas testosteroni |
| 4.2.1.84 | 100 | - |
+/- 15, substrate: 5-cyanovaleric acid, NilFe | Comamonas testosteroni |
| 4.2.1.84 | 110 | - |
+/- 10, substrate: 5-cyanovaleric acid, NilCo | Comamonas testosteroni |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.84 | (25R)-3beta-hydroxycholest-5-en-27-oate | - |
Comamonas testosteroni | ? | - |
? | |
| 4.2.1.84 | 5-cyanovaleric acid + H2O | NilFe and NilCo | Comamonas testosteroni | 6-amino-6-oxohexanoic acid | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.84 | tetramer | alpha2beta2, 2 * 22800 + 2 * 22700 | Comamonas testosteroni |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.84 | NHase | - |
Comamonas testosteroni |
| 4.2.1.84 | NilCo | - |
Comamonas testosteroni |
| 4.2.1.84 | NilFe | - |
Comamonas testosteroni |
| 4.2.1.84 | nitrile hydratase | - |
Comamonas testosteroni |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.84 | 6.9 | 7.2 | NilFe, NilCo | Comamonas testosteroni |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.84 | 0.002 | - |
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | +/- 0.0005, NilFe | Comamonas testosteroni | |
| 4.2.1.84 | 0.002 | - |
7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | +/- 0.0008, NilCo | Comamonas testosteroni | |
| 4.2.1.84 | 0.0025 | - |
KCN | +/- 0.001, NilCo | Comamonas testosteroni | |
| 4.2.1.84 | 0.003 | - |
NaNO2 | +/- 0.0005, NilCo | Comamonas testosteroni | |
| 4.2.1.84 | 0.003 | - |
KCN | +/- 0.0015, NilFe | Comamonas testosteroni | |
| 4.2.1.84 | 0.004 | - |
tertiary-butyl isonitrile | +/- 0.001, NilCo | Comamonas testosteroni | |
| 4.2.1.84 | 0.005 | - |
acrylonitrile | +/- 0.0005, NilCo | Comamonas testosteroni | |
| 4.2.1.84 | 0.007 | - |
acrylonitrile | +/- 0.001, NilFe | Comamonas testosteroni | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.84 | 0.003 | - |
+/- 0.001, NilCo | Comamonas testosteroni | KCN | |
| 4.2.1.84 | 0.003 | - |
+/- 0.001, NilCo | Comamonas testosteroni | 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | |
| 4.2.1.84 | 0.004 | - |
+/- 0.001, NilFe | Comamonas testosteroni | 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | |
| 4.2.1.84 | 0.005 | - |
+/- 0.001, NilFe | Comamonas testosteroni | KCN | |
| 4.2.1.84 | 0.005 | - |
+/- 0.001, NilFe | Comamonas testosteroni | 2-Nitrophenol | |
| 4.2.1.84 | 0.005 | - |
+/- 0.003, NilCo | Comamonas testosteroni | acrylonitrile | |
| 4.2.1.84 | 0.007 | - |
+/- 0.001, NilCo | Comamonas testosteroni | tertiary-butyl isonitrile | |
| 4.2.1.84 | 0.008 | - |
+/- 0.001, NilCo | Comamonas testosteroni | NaNO2 | |
| 4.2.1.84 | 0.0086 | - |
pH 7.9, NilCo | Comamonas testosteroni | 4-nitrophenol | |
| 4.2.1.84 | 0.009 | - |
+/- 0.002, NilFe | Comamonas testosteroni | acrylonitrile | |
| 4.2.1.84 | 0.01 | - |
+/- 0.002, NilFe | Comamonas testosteroni | 4-nitrophenol | |
| 4.2.1.84 | 0.01 | - |
+/- 0.002, NilFe | Comamonas testosteroni | 3-Nitrophenol | |
| 4.2.1.84 | 0.1 | - |
value above, NilFe | Comamonas testosteroni | tertiary-butyl isonitrile | |
| 4.2.1.84 | 0.3 | - |
+/- 0.1, NilCo | Comamonas testosteroni | Butyric acid | |
| 4.2.1.84 | 0.4 | - |
+/- 0.1, NilFe | Comamonas testosteroni | Butyric acid | |
| 4.2.1.84 | 0.5 | - |
value above, NilFe | Comamonas testosteroni | NaNO2 | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
