BRENDA - Enzyme Database show

Biochemical and spectroscopic characterization of an aldehyde oxidoreductase isolated from Desulfovibrio aminophilus

Thapper, A.; Rivas, M.G.; Brondino, C.D.; Ollivier, B.; Fauque, G.; Moura, I.; Moura, J.J.; J. Inorg. Biochem. 100, 44-50 (2006)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.3.1
Fe2+
contains two 2Fe-2S clusters permonomer, EPR spectroscopy of 2Fe-2S clusters
Desulfovibrio aminophilus
1.2.3.1
Mo5+
contains 0.7 Mo per monomer, EPR spectroscopy of Mo(V) centre
Desulfovibrio aminophilus
1.2.7.5
Fe2+
two(2Fe-2S) clusters per monomer
Desulfovibrio aminophilus
1.2.7.5
Molybdenum
molybdenum centre
Desulfovibrio aminophilus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.3.1
100000
-
2 * 100000, SDS-PAGE
Desulfovibrio aminophilus
1.2.3.1
200000
-
gel filtration
Desulfovibrio aminophilus
1.2.7.5
100000
-
2 * 100000, SDS-PAGE
Desulfovibrio aminophilus
1.2.7.5
200000
-
gel filtration
Desulfovibrio aminophilus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.3.1
Desulfovibrio aminophilus
-
strain DSM 12254, aminolytic strain performing thiosulfate dismutation
-
1.2.7.5
Desulfovibrio aminophilus
-
strain DSM12254
-
1.2.7.5
Desulfovibrio aminophilus DSM 12254
-
strain DSM12254
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.7.5
gel filtration
Desulfovibrio aminophilus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.3.1
0.3
-
pH 7.6, specific activity increase with substrate concentration reaching a maximum around 0.035 mM benzaldehyde and decreases above
Desulfovibrio aminophilus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.1
acetaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus
acetic acid + H2O2
-
-
-
?
1.2.3.1
acetaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus DSM 12254
acetic acid + H2O2
-
-
-
?
1.2.3.1
benzaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus
benzoic acid + H2O2
-
-
-
?
1.2.3.1
benzaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus DSM 12254
benzoic acid + H2O2
-
-
-
?
1.2.3.1
additional information
no substrate: xanthine, allopurinol, isoquinoline
675237
Desulfovibrio aminophilus
?
-
-
-
-
1.2.3.1
additional information
no substrate: xanthine, allopurinol, isoquinoline
675237
Desulfovibrio aminophilus DSM 12254
?
-
-
-
-
1.2.3.1
propionaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus
propionic acid + H2O2
-
-
-
?
1.2.3.1
propionaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus DSM 12254
propionic acid + H2O2
-
-
-
?
1.2.7.5
acetaldehyde + H2O + oxidized ferredoxin
-
675237
Desulfovibrio aminophilus
acetate + H+ + reduced ferredoxin
-
-
-
?
1.2.7.5
benzaldehyde + H2O + oxidized ferredoxin
-
675237
Desulfovibrio aminophilus
benzoic acid + H+ + reduced ferredoxin
-
-
-
?
1.2.7.5
additional information
no activity towards xanthine, allopurinol, and isoquinoline, inactive towards N-heterolytic compounds
675237
Desulfovibrio aminophilus
?
-
-
-
-
1.2.7.5
additional information
no activity towards xanthine, allopurinol, and isoquinoline, inactive towards N-heterolytic compounds
675237
Desulfovibrio aminophilus DSM 12254
?
-
-
-
-
1.2.7.5
propionaldehyde + H2O + oxidized ferredoxin
-
675237
Desulfovibrio aminophilus
propionate + H+ + reduced ferredoxin
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.3.1
dimer
2 * 100000, SDS-PAGE
Desulfovibrio aminophilus
1.2.7.5
homodimer
2 * 100000, SDS-PAGE
Desulfovibrio aminophilus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.2.3.1
Fe2+
contains two 2Fe-2S clusters permonomer, EPR spectroscopy of 2Fe-2S clusters
Desulfovibrio aminophilus
1.2.3.1
Mo5+
contains 0.7 Mo per monomer, EPR spectroscopy of Mo(V) centre
Desulfovibrio aminophilus
1.2.7.5
Fe2+
two(2Fe-2S) clusters per monomer
Desulfovibrio aminophilus
1.2.7.5
Molybdenum
molybdenum centre
Desulfovibrio aminophilus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.3.1
100000
-
2 * 100000, SDS-PAGE
Desulfovibrio aminophilus
1.2.3.1
200000
-
gel filtration
Desulfovibrio aminophilus
1.2.7.5
100000
-
2 * 100000, SDS-PAGE
Desulfovibrio aminophilus
1.2.7.5
200000
-
gel filtration
Desulfovibrio aminophilus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.7.5
gel filtration
Desulfovibrio aminophilus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.3.1
0.3
-
pH 7.6, specific activity increase with substrate concentration reaching a maximum around 0.035 mM benzaldehyde and decreases above
Desulfovibrio aminophilus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.3.1
acetaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus
acetic acid + H2O2
-
-
-
?
1.2.3.1
acetaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus DSM 12254
acetic acid + H2O2
-
-
-
?
1.2.3.1
benzaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus
benzoic acid + H2O2
-
-
-
?
1.2.3.1
benzaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus DSM 12254
benzoic acid + H2O2
-
-
-
?
1.2.3.1
additional information
no substrate: xanthine, allopurinol, isoquinoline
675237
Desulfovibrio aminophilus
?
-
-
-
-
1.2.3.1
additional information
no substrate: xanthine, allopurinol, isoquinoline
675237
Desulfovibrio aminophilus DSM 12254
?
-
-
-
-
1.2.3.1
propionaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus
propionic acid + H2O2
-
-
-
?
1.2.3.1
propionaldehyde + H2O + O2
-
675237
Desulfovibrio aminophilus DSM 12254
propionic acid + H2O2
-
-
-
?
1.2.7.5
acetaldehyde + H2O + oxidized ferredoxin
-
675237
Desulfovibrio aminophilus
acetate + H+ + reduced ferredoxin
-
-
-
?
1.2.7.5
benzaldehyde + H2O + oxidized ferredoxin
-
675237
Desulfovibrio aminophilus
benzoic acid + H+ + reduced ferredoxin
-
-
-
?
1.2.7.5
additional information
no activity towards xanthine, allopurinol, and isoquinoline, inactive towards N-heterolytic compounds
675237
Desulfovibrio aminophilus
?
-
-
-
-
1.2.7.5
additional information
no activity towards xanthine, allopurinol, and isoquinoline, inactive towards N-heterolytic compounds
675237
Desulfovibrio aminophilus DSM 12254
?
-
-
-
-
1.2.7.5
propionaldehyde + H2O + oxidized ferredoxin
-
675237
Desulfovibrio aminophilus
propionate + H+ + reduced ferredoxin
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.3.1
dimer
2 * 100000, SDS-PAGE
Desulfovibrio aminophilus
1.2.7.5
homodimer
2 * 100000, SDS-PAGE
Desulfovibrio aminophilus