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Literature summary extracted from

  • Casella, L.; Gullotti, M.; Monzani, E.; Santagostini, L.; Zoppellaro, G.; Sakurai, T.
    Enzymatic and spectroscopic studies on the activation or inhibition effects by substituted pHenolic compounds in the oxidation of aryldiamines and catechols catalyzed by Rhus vernicifera laccase (2006), J. Inorg. Biochem., 100, 2127-2139.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.2 2-chlorophenol activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
1.10.3.2 2-Fluorophenol activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
1.10.3.2 4-Chlorophenol activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
1.10.3.2 4-Fluorophenol activates, effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 4-hydroxyphenylacetic acid effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
1.10.3.2 4-hydroxyphenylpropionic acid effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum
1.10.3.2 4-nitrophenol effects on enzyme kinetics with substrate N,N-dimethyl-4-phenylenediamine, overview Toxicodendron vernicifluum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information kinetics in absence and presence of acidic and halophenols, overview Toxicodendron vernicifluum
1.10.3.2 2.3 2.5 N,N-dimethyl-4-phenylenediamine pH 7.0, 20°C Toxicodendron vernicifluum
1.10.3.2 3.3 3.8 N,N-dimethyl-4-phenylenediamine pH 7.0, 25°C Toxicodendron vernicifluum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ copper-containing enzyme, trinuclear Cu cluster upon interaction with o-fluorophenol, overview Toxicodendron vernicifluum

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Toxicodendron vernicifluum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 4-tert-butylcatechol + O2
-
 Toxicodendron vernicifluum ?
-
 ?
1.10.3.2 N,N-dimethyl-4-phenylenediamine + O2
-
 Toxicodendron vernicifluum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 25
-
 assay at Toxicodendron vernicifluum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 181 186 N,N-dimethyl-4-phenylenediamine pH 7.0, 20°C Toxicodendron vernicifluum
1.10.3.2 350 443 N,N-dimethyl-4-phenylenediamine pH 7.0, 25°C Toxicodendron vernicifluum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 7
-
 assay at Toxicodendron vernicifluum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 4.3 9
-
 Toxicodendron vernicifluum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.10.3.2 additional information
-
 additional information inhibition kinetics with acidic phenols, overview Toxicodendron vernicifluum