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Literature summary extracted from

  • Huembelin, M.; Griesser, V.; Keller, T.; Schurter, W.; Haiker, M.; Hohmann, H.P.; Ritz, H.; Richter, G.; Bacher, A.; Van Loon, A.P.G.M.
    GTP cyclohydrolase II and 3,4-dihydroxy-2-butanone 4-phosphate synthase are rate-limiting enzymes in riboflavin synthesis of an industrial Bacillus subtilis strain used for riboflavin production (1999), J. Indust. Microbiol. Biotechnol., 22, 1-7.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.5.4.25 synthesis the enzyme is essential for industrial riboflavin production by Bacillus subtilis overproducing strains, overview Bacillus subtilis
4.1.99.12 synthesis the enzyme is essential for industrial riboflavin production by Bacillus subtilis overproducing strains, overview Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.25 overexpression in strain 1012 by gene insertion in the sacB locus Bacillus subtilis
4.1.99.12 overexpression in strain 1012 by gene insertion in the sacB locus Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.25 additional information an additional single copy of the ribA gene introduced into the sacBlocus of the riboflavin production strain and constitutive expression from the medium strength vegI promoter leads to improved riboflavin titers and yields of riboflavin on glucose of up to 25%, strain VB2XL1, both enzymatic activities of RibA, the 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and the GTP cyclohydrolase II activity of the C-terminal domain, are necessary for the improved riboflavin productivity, method, overview Bacillus subtilis
4.1.99.12 additional information an additional single copy of the ribA gene introduced into the sacBlocus of the riboflavin production strain and constitutive expression from the medium strength vegI promoter leads to improved riboflavin titers and yields of riboflavin on glucose of up to 25%, strain VB2XL1, both enzymatic activities of RibA, the 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and the GTP cyclohydrolase II activity of the C-terminal domain, are necessary for the improved riboflavin productivity, method, overview Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.4.25 EDTA
-
 Bacillus subtilis
4.1.99.12 EDTA
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.25 Mg2+
-
 Bacillus subtilis
4.1.99.12 Mg2+
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.25 GTP + H2O Bacillus subtilis
-
 formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
 ?
3.5.4.25 additional information Bacillus subtilis RibA is the rate limiting enzyme in an industrial riboflavin producing strain ?
-
 ?
4.1.99.12 D-ribulose 5-phosphate Bacillus subtilis rate-limiting step in riboflavin biosynthesis formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
4.1.99.12 additional information Bacillus subtilis RibA is the rate limiting enzyme in an industrial riboflavin producing strain ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.25 Bacillus subtilis
-
 gene ribA
-
4.1.99.12 Bacillus subtilis
-
 gene ribA
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.25 GTP + 4 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + 2 phosphate RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity, EC 4.1.99.12, located in the N-terminal half of the protein and GTP cyclohydrolase II activity of the C-terminal domain, overview Bacillus subtilis
a
4.1.99.12 D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and GTP cyclohydrolase II activity, EC 3.5.4.25, of the C-terminal domain, overview Bacillus subtilis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.25 GTP + H2O
-
 Bacillus subtilis formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate
-
 ?
3.5.4.25 additional information RibA is the rate limiting enzyme in an industrial riboflavin producing strain Bacillus subtilis ?
-
 ?
3.5.4.25 additional information RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity, EC 4.1.99.12, located in the N-terminal half of the protein and GTP cyclohydrolase II activity of the C-terminal domain, overview Bacillus subtilis ?
-
 ?
4.1.99.12 D-ribulose 5-phosphate
-
 Bacillus subtilis formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
4.1.99.12 D-ribulose 5-phosphate rate-limiting step in riboflavin biosynthesis Bacillus subtilis formate + L-3,4-dihydroxy-2-butanone-4-phosphate
-
 ?
4.1.99.12 additional information RibA is the rate limiting enzyme in an industrial riboflavin producing strain Bacillus subtilis ?
-
 ?
4.1.99.12 additional information RibA is a bifunctional enzyme possessing 3,4-dihydroxy-2-butanone 4-phosphate synthase activity located in the N-terminal half of the protein and GTP cyclohydrolase II activity, EC 3.5.4.25, of the C-terminal domain, overview Bacillus subtilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.4.25 More cf. EC 4.1.99.12 Bacillus subtilis
3.5.4.25 ribA
-
 Bacillus subtilis
4.1.99.12 More cf. EC 3.5.4.25 Bacillus subtilis
4.1.99.12 ribA
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.4.25 37
-
 assay at Bacillus subtilis
4.1.99.12 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.4.25 8.5
-
 assay at Bacillus subtilis
4.1.99.12 8
-
 assay at Bacillus subtilis