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Literature summary extracted from

  • He, Z.; Parales, R.E.; Spain, J.C.; Johnson, G.R.
    Novel organization of catechol meta pathway genes in the nitrobenzene degrader Comamonas sp. JS765 and its evolutionary implication (2007), J. Ind. Microbiol. Biotechnol., 34, 99-104.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.32 additional information HMSD in crude extracts prepared from pDTG912/B shows higher activity in potassium phosphate buffer than in Tris-HCl buffer (50 mM) Comamonas sp. JS765

Application

EC Number Application Comment Organism
1.2.1.32 additional information codG encoding 2-hydroxymuconic semialdehyde dehydrogenase shows a higher degree of similarity to those genes in classical bacteria Comamonas sp. JS765

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.32 expressed in Escherichia coli carrying pDTG912, pDTG912/A, and pDTG912/B Comamonas sp. JS765
1.13.11.2 expressed in Escherichia coli strains JM109 and DH5alpha Comamonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.32 52300
-
 sequence analysis Comamonas sp. JS765

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.32 Comamonas sp. JS765
-
-
-
1.13.11.2 Comamonas sp.
-
 JS765
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.2 additional information
-
 pDTG912 and pDTG901 are deletion derivatives of the cosmid pDTG900. pDTG912/A and pDTG912/B are subclones of pDTG912 in pK18. Activity of the enzyme related to pDTG912 = 0.012 micromol/min/mg, activity of the enzyme related to pDTG901 = 0.56 micromol/min/mg, no detectable activity of the enzyme related to pDTG912/A, no detactable activity of the enzyme related to pDTG912/B Comamonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.32 2-aminomuconic acid semialdehyde + NAD+ + H2O
-
 Comamonas sp. JS765 2-aminomuconate + NADH + H+
-
 ?
1.2.1.32 2-hydroxymuconic acid semialdehyde + NAD+ + H2O
-
 Comamonas sp. JS765 2-hydroxymuconate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.32 2-hydroxymuconic semialdehyde dehydrogenase
-
 Comamonas sp. JS765
1.2.1.32 HMSD-2
-
 Comamonas sp. JS765
1.13.11.2 C23o
-
 Comamonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.32 8.5
-
-
 Comamonas sp. JS765

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.2.1.32 Comamonas sp. JS765 calculated
-
 5.61