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Literature summary extracted from

  • Furukawa, K.; Suyama, A.; Tsuboi, Y.; Futagami, T.; Goto, M.
    Biochemical and molecular characterization of a tetrachloroethene dechlorinating Desulfitobacterium sp. strain Y51: a review (2005), J. Ind. Microbiol. Biotechnol., 32, 534-541.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.99.5 cis-1,2-dichloroethene significant growth inhibition of strain in presence of cis-1,2-dichloroethene, during growth in presence of cis-1,2-dichloroethene, the enzyme gene cluster is deleted with high frequency Desulfitobacterium sp.
1.21.99.5 propyliodide 25 mM, 13% residual activity. When exposed to light, the activity is restored to 59% after 6 h and 90% after 24 h Desulfitobacterium sp.
1.21.99.5 sulfite 20 mM, residual activity 53% Desulfitobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.99.5 0.105
-
 tetrachloroethene pH 7.5, 37°C Desulfitobacterium sp.
1.21.99.5 0.125
-
 hexachloroethane
-
 Desulfitobacterium sp.
1.21.99.5 0.336
-
 1,1,2,2-tetrachloroethane
-
 Desulfitobacterium sp.
1.21.99.5 0.535
-
 1,1,2-trichloroethene pH 7.5, 37°C Desulfitobacterium sp.
1.21.99.5 0.6199
-
 pentachloroethane
-
 Desulfitobacterium sp.
1.21.99.5 0.773
-
 1,1,1,2-tetrachloroethane
-
 Desulfitobacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.21.99.5 periplasm
-
 Desulfitobacterium sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.99.5 58000
-
 x * 58000, SDS-PAGE of mature protein Desulfitobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.21.99.5 Desulfitobacterium sp.
-
-
-
1.21.99.5 Desulfitobacterium sp. Y51
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.21.99.5 proteolytic modification x * 61000 Da, unprocessed protein, x * 58000, mature protein Desulfitobacterium sp.

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.21.99.5 in presence of 25 mM propyliodide, 13% residual activity. When exposed to light, the activity is restored to 59% after 6 h and 90% after 24 h Desulfitobacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.99.5 113.6
-
 pH 7.5, 37°C Desulfitobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.99.5 1,1,1,2,2,3,3-heptachloropropane + reduced acceptor
-
 Desulfitobacterium sp. 1,1,2,3,3,3-hexachloroprop-1-ene + chloride + acceptor
-
 ?
1.21.99.5 1,1,1,2,2,3,3-heptachloropropane + reduced acceptor
-
 Desulfitobacterium sp. Y51 1,1,2,3,3,3-hexachloroprop-1-ene + chloride + acceptor
-
 ?
1.21.99.5 1,1,1,2-tetrachloroethane + reduced acceptor
-
 Desulfitobacterium sp. 1,1-dichlorethene + chloride + acceptor
-
 ?
1.21.99.5 1,1,1,2-tetrachloroethane + reduced acceptor
-
 Desulfitobacterium sp. Y51 1,1-dichlorethene + chloride + acceptor
-
 ?
1.21.99.5 1,1,2,2-tetrachloroethane + reduced acceptor
-
 Desulfitobacterium sp. cis-1,2-dichlorethene + chloride + acceptor
-
 ?
1.21.99.5 1,1,2,2-tetrachloroethane + reduced acceptor
-
 Desulfitobacterium sp. Y51 cis-1,2-dichlorethene + chloride + acceptor
-
 ?
1.21.99.5 1,1,2-trichloroethene + reduced acceptor
-
 Desulfitobacterium sp. cis-1,2-dichlorethene + chloride + acceptor
-
 ?
1.21.99.5 1,1,2-trichloroethene + reduced acceptor
-
 Desulfitobacterium sp. Y51 cis-1,2-dichlorethene + chloride + acceptor
-
 ?
1.21.99.5 hexachloroethane + reduced acceptor
-
 Desulfitobacterium sp. cis-1,2-dichlorethene + chloride + acceptor
-
 ?
1.21.99.5 pentachloroethane + reduced acceptor
-
 Desulfitobacterium sp. cis-1,2-dichlorethene + chloride + acceptor
-
 ?
1.21.99.5 tetrachloroethene + reduced acceptor
-
 Desulfitobacterium sp. trichloroethene + chloride + acceptor
-
 ?
1.21.99.5 tetrachloroethene + reduced acceptor
-
 Desulfitobacterium sp. Y51 trichloroethene + chloride + acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.99.5 ? x * 58000, SDS-PAGE of mature protein Desulfitobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.99.5 37
-
-
 Desulfitobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.99.5 7 7.5
-
 Desulfitobacterium sp.