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Literature summary extracted from

  • Weng, M.; Pfeifer, O.; Krauss, S.; Lingens, F.; van Pee, K.H.
    Purification, characterization and comparison of two non-haem bromoperoxidases from Streptomyces aureofaciens ATCC 10762 (1991), J. Gen. Microbiol., 137, 2539-2546.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.18 31000
-
 2 * 31000, BPO 1, SDS-PAGE Kitasatospora aureofaciens
1.11.1.18 31000
-
 3 * 31000, BPO 2, SDS-PAGE Kitasatospora aureofaciens
1.11.1.18 65000
-
 BPO 1, gel filtration Kitasatospora aureofaciens
1.11.1.18 90000
-
 BPO 2, gel filtration Kitasatospora aureofaciens

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.18 Kitasatospora aureofaciens
-
 ATCC 10762
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.18 BPO 1 Kitasatospora aureofaciens
1.11.1.18 BPO 2 Kitasatospora aureofaciens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.18 15.4
-
 BPO 2 Kitasatospora aureofaciens
1.11.1.18 45.23
-
 BPO 1 Kitasatospora aureofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.18 Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione i.e. monochlorodimedone Kitasatospora aureofaciens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.18 dimer 2 * 31000, BPO 1, SDS-PAGE Kitasatospora aureofaciens
1.11.1.18 trimer 3 * 31000, BPO 2, SDS-PAGE Kitasatospora aureofaciens

Synonyms

EC Number Synonyms Comment Organism
1.11.1.18 BPO 1
-
 Kitasatospora aureofaciens
1.11.1.18 BPO 2
-
 Kitasatospora aureofaciens
1.11.1.18 non-haem bromoperoxidase BPO 1
-
 Kitasatospora aureofaciens
1.11.1.18 non-haem bromoperoxidase BPO 2
-
 Kitasatospora aureofaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.18 70
-
 4 h, 36% loss of activity, BPO 2 Kitasatospora aureofaciens
1.11.1.18 70
-
 4 h, no inactivation of BPO 1 Kitasatospora aureofaciens
1.11.1.18 80
-
 5 min, 50% loss of ativity, BPO 2 Kitasatospora aureofaciens
1.11.1.18 80
-
 5 min, no inactivation of BPO 1 Kitasatospora aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.18 4.5
-
 1 mM sodium acetate buffer, BPO 2 Kitasatospora aureofaciens
1.11.1.18 5.5
-
 1 mM sodium acetate buffer, BPO 1 Kitasatospora aureofaciens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.18 Kitasatospora aureofaciens isoelectric focusing, BPO 2
-
 3.5
1.11.1.18 Kitasatospora aureofaciens isoelectric focusing, BPO 1
-
 4.5