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Literature summary extracted from

  • van der Rest, B.; Danoun, S.; Boudet, A.M.; Rochange, S.F.
    Down-regulation of cinnamoyl-CoA reductase in tomato (Solanum lycopersicum L.) induces dramatic changes in soluble phenolic pools (2006), J. Exp. Bot., 57, 1399-1411.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.44 for generation of RNAi constructs and for tomato transformation through Agrobacterium tumefaciens, into the pGEX4T-1 vector for expression in Escherichia coli BL21 cells Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.44 36800
-
predicted molecular mass for CCR1 and CCR2 Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.44 cinnamaldehyde + CoA + NADP+ Solanum lycopersicum
-
cinnamoyl-CoA + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Solanum lycopersicum Q4U1I4
-
-
1.2.1.44 Solanum lycopersicum Q4U1I5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44 recombinant proteins are purified on a glutathione resin Solanum lycopersicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.44 flower
-
Solanum lycopersicum
-
1.2.1.44 leaf
-
Solanum lycopersicum
-
1.2.1.44 stem
-
Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 caffeoyl-CoA + NADPH + H+ activity assay Solanum lycopersicum caffealdehyde + CoA + NADP+
-
?
1.2.1.44 cinnamaldehyde + CoA + NADP+
-
Solanum lycopersicum cinnamoyl-CoA + NADPH + H+
-
?
1.2.1.44 coumaroyl-CoA + NADPH + H+ activity assay Solanum lycopersicum coumaraldehyde + CoA + NADP+
-
?
1.2.1.44 feruloyl-CoA + NADPH + H+ activity assay Solanum lycopersicum coniferaldehyde + CoA + NADP+
-
?
1.2.1.44 sinapoyl-CoA + NADPH + H+ activity assay Solanum lycopersicum sinapaldehyde + CoA + NADP+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 CCR1
-
Solanum lycopersicum
1.2.1.44 CCR2
-
Solanum lycopersicum
1.2.1.44 cinnamoyl-CoA reductase
-
Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.44 0.33
-
caffeoyl-CoA CCR1 Solanum lycopersicum
1.2.1.44 0.43
-
coumaroyl-CoA CCR1 Solanum lycopersicum
1.2.1.44 0.96
-
sinapoyl-CoA CCR1 Solanum lycopersicum
1.2.1.44 1.6
-
feruloyl-CoA CCR2 Solanum lycopersicum
1.2.1.44 14.2
-
feruloyl-CoA CCR1 Solanum lycopersicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.44 6.2
-
activity assay Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.44 NADPH
-
Solanum lycopersicum