EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.44 | for generation of RNAi constructs and for tomato transformation through Agrobacterium tumefaciens, into the pGEX4T-1 vector for expression in Escherichia coli BL21 cells | Solanum lycopersicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.44 | 36800 | - |
predicted molecular mass for CCR1 and CCR2 | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.44 | cinnamaldehyde + CoA + NADP+ | Solanum lycopersicum | - |
cinnamoyl-CoA + NADPH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.44 | Solanum lycopersicum | Q4U1I4 | - |
- |
1.2.1.44 | Solanum lycopersicum | Q4U1I5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.44 | recombinant proteins are purified on a glutathione resin | Solanum lycopersicum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.44 | flower | - |
Solanum lycopersicum | - |
1.2.1.44 | leaf | - |
Solanum lycopersicum | - |
1.2.1.44 | stem | - |
Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.44 | caffeoyl-CoA + NADPH + H+ | activity assay | Solanum lycopersicum | caffealdehyde + CoA + NADP+ | - |
? | |
1.2.1.44 | cinnamaldehyde + CoA + NADP+ | - |
Solanum lycopersicum | cinnamoyl-CoA + NADPH + H+ | - |
? | |
1.2.1.44 | coumaroyl-CoA + NADPH + H+ | activity assay | Solanum lycopersicum | coumaraldehyde + CoA + NADP+ | - |
? | |
1.2.1.44 | feruloyl-CoA + NADPH + H+ | activity assay | Solanum lycopersicum | coniferaldehyde + CoA + NADP+ | - |
? | |
1.2.1.44 | sinapoyl-CoA + NADPH + H+ | activity assay | Solanum lycopersicum | sinapaldehyde + CoA + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.44 | CCR1 | - |
Solanum lycopersicum |
1.2.1.44 | CCR2 | - |
Solanum lycopersicum |
1.2.1.44 | cinnamoyl-CoA reductase | - |
Solanum lycopersicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.44 | 0.33 | - |
caffeoyl-CoA | CCR1 | Solanum lycopersicum | |
1.2.1.44 | 0.43 | - |
coumaroyl-CoA | CCR1 | Solanum lycopersicum | |
1.2.1.44 | 0.96 | - |
sinapoyl-CoA | CCR1 | Solanum lycopersicum | |
1.2.1.44 | 1.6 | - |
feruloyl-CoA | CCR2 | Solanum lycopersicum | |
1.2.1.44 | 14.2 | - |
feruloyl-CoA | CCR1 | Solanum lycopersicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.44 | 6.2 | - |
activity assay | Solanum lycopersicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.44 | NADPH | - |
Solanum lycopersicum |