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Literature summary extracted from

  • Su, J.; Hirji, R.; Zhang, L.; He, C.; Selvaraj, G.; Wu, R.
    Evaluation of the stress-inducible production of choline oxidase in transgenic rice as a strategy for producing the stress-protectant glycine betaine (2006), J. Exp. Bot., 57, 1129-1135.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.17 overexpression of the enzyme, fused to a chloroplast-transit peptide from Pisum sativum, in transgenic Oryza sativa plants using the Agrobacterium tumefaciens transfection system Arthrobacter pascens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.17 additional information evaluation of the stress-inducible production of Arthrobacter pascens choline oxidase in transgenic rice as a strategy for producing the stress-protectant glycine betaine, stress induction by 150 mM NaCl, biomass production in wild-type and transgenic plants Arthrobacter pascens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.17 choline + O2 Arthrobacter pascens
-
 betaine aldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Arthrobacter pascens
-
 gene COX
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.17 recombinant enzyme partially from stressed and non-stressed transgenic rice plant leaves by ammonium sulfate fractionation and desalting gel filtration Arthrobacter pascens

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.17 choline + O2 = betaine aldehyde + H2O2 the enzyme catalyzes the oxidation of choline to glycine betaine via the intermediate betaine aldehyde Arthrobacter pascens
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.17 additional information
-
 activity measurement in a coupled assay with NAD+-dependent betaine aldehyde dehydrogenase, BADH Arthrobacter pascens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 choline + O2
-
 Arthrobacter pascens betaine aldehyde + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.17 COX
-
 Arthrobacter pascens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.17 22
-
 assay at Arthrobacter pascens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.17 8
-
 assay at Arthrobacter pascens