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Literature summary extracted from

  • Satoh, M.; Iwahori, T.; Sugawara, N.; Yamazaki, M.
    Liver argininosuccinate synthase binds to bacterial lipopolysaccharides and lipid A and inactivates their biological activities (2006), J. Endotoxin Res., 12, 21-38.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.4.5 medicine argininosuccinate synthetase is inhibited by endotoxin, implying that bacterial products might potentially regulate the arginine metabolism in the host and perturb NO synthesis Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.5 diphosphoryl lipid A (DPL) DPL from Escherichia coli, inhibits argininosuccinate synthetase in a dose-dependent manner, concentration for half inhibition of argininosuccinate synthetase enzyme activity: 0.039 mg/ml Mus musculus
6.3.4.5 monophosphoryl lipid A (MPL) MPL from Escherichia coli, inhibits argininosuccinate synthetase in a dose-dependent manner, concentration for half inhibition of argininosuccinate synthetase enzyme activity: 0.270 mg/ml Mus musculus
6.3.4.5 R-lipopolysaccharide (LPS) rough-type LPS from Escherichia coli, inhibits argininosuccinate synthetase in a dose-dependent manner, concentration for half inhibition of argininosuccinate synthetase enzyme activity: 0.230 mg/ml Mus musculus
6.3.4.5 S-lipopolysaccharide (LPS) smooth-type-LPS from Escherichia coli, inhibits argininosuccinate synthetase in a dose-dependent manner, concentration for half inhibition of argininosuccinate synthetase enzyme activity: 0.024 mg/ml Mus musculus
6.3.4.5 sodium 2-deoxy-2-(3S-(9-phenyl-nonanoyl-oxy) tetradecanoyl) amino-3-O-(9-phenyl-nonanoyl)-D-glucopyranose 4-sulfate synthesized lipid A analog, inhibits argininosuccinate synthetase in a dose-dependent manner, concentration for half inhibition of argininosuccinate synthetase enzyme activity: 0.0065 mg/ml Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.4.5 Mg2+
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.5 45000
-
 SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.5 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.5 analysis by native gel electrophoresis shows that argininosuccinate synthetase can bind bacterial lipopolysaccharide and lipid A Mus musculus
6.3.4.5 argininosuccinate synthetase is purified from mouse liver. Livers are excised from 20 male mice (C3H/He strain, age 7-8 weeks) and homogenized in 100 mM Tris-HCl (pH 7.5) containing 1 mM citrulline and 1 mM aspartic acid. Homogenate is centrifuged. After salting-out with (NH4)2SO4. The sample is subjected to anion-exchange chromatography on DE-52. Concentrated samples are loaded on a column of Sephacryl S-300 HR. The collected first peak fractions are dialyzed and subjected to anion-exchange chromatography on UNO S-1 Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.5 ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate it is found that argininosuccinate synthetase is the major lipid A-interacting protein in liver. Argininosuccinate synthetase also inhibits the biological activities of natural lipid A and rough-type lipopolysaccharide and but not smooth-type lipopolysaccharide. Argininosuccinate synthetase enzyme activity is inhibited by lipid A and rough-type lipopolysaccharide and smooth-type lipopolysaccharide. Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.4.5 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.5 0.14
-
 purification step: liver homogenate supernatant Mus musculus
6.3.4.5 0.27
-
 purification step: 45-55% saturated ammonium sulfate precipitate Mus musculus
6.3.4.5 0.99
-
 purification step: DE-52 fraction Mus musculus
6.3.4.5 1.39
-
 purification step: S-300 fraction Mus musculus
6.3.4.5 5.16
-
 purification step: UNO S-1 fraction Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.5 ATP + L-citrulline + L-aspartate
-
 Mus musculus AMP + diphosphate + L-argininosuccinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.5 Argininosuccinate synthetase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.4.5 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.4.5 7.5
-
 assay at Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.4.5 additional information
-
 substrate: diphosphoryl lipid A, IC50: 0.039 mg/ml Mus musculus additional information
6.3.4.5 additional information
-
 substrate: monophosphoryl lipid A, IC50: 0.270 mg/ml Mus musculus additional information
6.3.4.5 additional information
-
 substrate: R-lipopolysaccharide, IC50: 0.230 mg/ml Mus musculus additional information
6.3.4.5 additional information
-
 substrate: S-lipopolysaccharide, IC50: 0.024 mg/ml Mus musculus additional information
6.3.4.5 additional information
-
 substrate: sodium 2-deoxy-2-(3S-(9-phenyl-nonanoyl-oxy) tetradecanoyl) amino-3-O-(p-phenyl-nonanoyl)-D-glucopyranose 4-sulfate (ONO-4007), IC50: 0.0065 mg/ml Mus musculus additional information