EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.2.8 | analysis | the branched bi-enzyme system with xanthine oxidase is an important biochemical system to evaluate the efficiency of the anticancer drug 6-mercaptopurine, overview | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.8 | expressed in Escherichia coli strain BL21(DE3) | Homo sapiens |
2.4.2.8 | gene HGPRT, expression in Escherichia coli strain BL21(DE3) | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | additional information | - |
additional information | kinetic analysis of the branched bi-enzyme system, detailed overview | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.17.3.2 | extracellular | - |
Homo sapiens | - |
- |
2.4.2.8 | cytoplasm | - |
Homo sapiens | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.2.8 | Mg2+ | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.3.2 | 6-mercaptopurine + 2 H2O + 2 O2 | Bos taurus | - |
6-thiouric acid + 2 H2O2 | - |
? | |
1.17.3.2 | 6-mercaptopurine + 2 H2O + 2 O2 | Homo sapiens | an anticancer prodrug, no activation by xanthine oxidase but conversion to the inactive metabolite 6-thiouric acid, catabolism, overview | 6-thiouric acid + 2 H2O2 | - |
? | |
1.17.3.2 | xanthine + H2O + O2 | Homo sapiens | - |
urate + H2O2 | - |
? | |
1.17.3.2 | xanthine + H2O + O2 | Bos taurus | - |
urate + H2O2 | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
IMP + diphosphate | - |
? | |
2.4.2.8 | additional information | Homo sapiens | the enzyme is organized in a branched bi-enzyme system with xanthine oxidase, EC 1.17.3.2 | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.3.2 | Bos taurus | - |
- |
- |
1.17.3.2 | Homo sapiens | - |
- |
- |
2.4.2.8 | Homo sapiens | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.17.3.2 | commercial preparation | - |
Bos taurus | - |
1.17.3.2 | JURKAT cell | - |
Homo sapiens | - |
1.17.3.2 | lymphocyte | - |
Homo sapiens | - |
1.17.3.2 | milk | - |
Bos taurus | - |
1.17.3.2 | plasma | - |
Homo sapiens | - |
2.4.2.8 | acute lymphoblastic leukemia cell | - |
Homo sapiens | - |
2.4.2.8 | JURKAT cell | - |
Homo sapiens | - |
2.4.2.8 | JURKAT cell | a T-ALL cell line | Homo sapiens | - |
2.4.2.8 | lymphocyte | immature | Homo sapiens | - |
2.4.2.8 | T-ALL cell | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | additional information | - |
rate of extracellular catabolism of 6-mercaptopurine by XOD | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.3.2 | 6-mercaptopurine + 2 H2O + 2 O2 | - |
Homo sapiens | 6-thiouric acid + 2 H2O2 | - |
? | |
1.17.3.2 | 6-mercaptopurine + 2 H2O + 2 O2 | - |
Bos taurus | 6-thiouric acid + 2 H2O2 | - |
? | |
1.17.3.2 | 6-mercaptopurine + 2 H2O + 2 O2 | an anticancer prodrug, no activation by xanthine oxidase but conversion to the inactive metabolite 6-thiouric acid, catabolism, overview | Homo sapiens | 6-thiouric acid + 2 H2O2 | - |
? | |
1.17.3.2 | additional information | evaluation of a HPLC detection method for enzyme reaction products, overview | Homo sapiens | ? | - |
? | |
1.17.3.2 | additional information | evaluation of a HPLC detection method for enzyme reaction products, overview | Bos taurus | ? | - |
? | |
1.17.3.2 | xanthine + H2O + O2 | - |
Homo sapiens | urate + H2O2 | - |
? | |
1.17.3.2 | xanthine + H2O + O2 | - |
Bos taurus | urate + H2O2 | - |
? | |
2.4.2.8 | 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | a thiopurine antimetabolite | Homo sapiens | thioinosinic monophosphate + diphosphate | a therapeutically active metabolite | ? | |
2.4.2.8 | 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 6-thioinosine monophosphate + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
? | |
2.4.2.8 | additional information | the enzyme is organized in a branched bi-enzyme system with xanthine oxidase, EC 1.17.3.2 | Homo sapiens | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.17.3.2 | XOD | - |
Homo sapiens |
1.17.3.2 | XOD | - |
Bos taurus |
2.4.2.8 | HGPRT | - |
Homo sapiens |
2.4.2.8 | hypoxanthine-guanine phosphoribosyltransferase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | 37 | - |
assay at | Homo sapiens |
1.17.3.2 | 37 | - |
assay at | Bos taurus |
2.4.2.8 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.17.3.2 | 7.4 | - |
assay at | Homo sapiens |
1.17.3.2 | 7.4 | - |
assay at | Bos taurus |
2.4.2.8 | 7.4 | - |
assay at | Homo sapiens |