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Literature summary extracted from

  • Papinutti, L.; Martinez, M.J.
    Production and characterization of laccase and manganese peroxidase from the ligninolytic fungus Fomes sclerodermeus (2006), J. Chem. Technol. Biotechnol., 81, 1064-1070.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.2 Cu2+ stimulates, induction Fomes sclerodermeus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information both isozymes show Michaelis-Menten kinetics, overview Fomes sclerodermeus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 67000
-
 x * 67000, both isozymes, SDS-PAGE Fomes sclerodermeus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Fomes sclerodermeus
-
 two isozymes Lac I and Lac II
-
1.10.3.2 Fomes sclerodermeus BAFC 2752
-
 two isozymes Lac I and Lac II
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein 3% N-linked carbohydrate content for both isozymes Fomes sclerodermeus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native isozymes Lac I and II by anion exchange chromatography and gel filtration, overview Fomes sclerodermeus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 additional information enzyme production in presence or absence of exogenous copper, overview Fomes sclerodermeus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 additional information
-
-
 Fomes sclerodermeus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Fomes sclerodermeus ?
-
 ?
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Fomes sclerodermeus BAFC 2752 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 preferred substrate Fomes sclerodermeus 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O i.e. coerulignone ?
1.10.3.2 2,6-dimethoxyphenol + O2 preferred substrate Fomes sclerodermeus BAFC 2752 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O i.e. coerulignone ?
1.10.3.2 ferulic acid + O2
-
 Fomes sclerodermeus ?
-
 ?
1.10.3.2 ferulic acid + O2
-
 Fomes sclerodermeus BAFC 2752 ?
-
 ?
1.10.3.2 vanillic acid + O2
-
 Fomes sclerodermeus ?
-
 ?
1.10.3.2 vanillic acid + O2
-
 Fomes sclerodermeus BAFC 2752 ?
-
 ?
1.10.3.2 veratric acid + O2
-
 Fomes sclerodermeus ?
-
 ?
1.10.3.2 veratric acid + O2
-
 Fomes sclerodermeus BAFC 2752 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 67000, both isozymes, SDS-PAGE Fomes sclerodermeus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 182
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, isozyme Lac II Fomes sclerodermeus
1.10.3.2 199
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.0, isozyme Lac I Fomes sclerodermeus
1.10.3.2 206
-
 2,6-dimethoxyphenol pH 5.0, isozyme Lac I Fomes sclerodermeus
1.10.3.2 218
-
 2,6-dimethoxyphenol pH 5.0, isozyme Lac II Fomes sclerodermeus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5
-
 assay at Fomes sclerodermeus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Fomes sclerodermeus Lac I, isoelectric focusing
-
 3.41
1.10.3.2 Fomes sclerodermeus Lac II, isoelectric focusing
-
 3.48