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Literature summary extracted from

  • Park, H.Y.; Park, C.S.; Kim, H.J.; Oh, D.K.
    Substrate specificity of a galactose 6-phosphate isomerase from Lactococcus lactis that produces d-allose from d-psicose (2007), J. Biotechnol., 132, 88-95.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.26 overexpression in Escherichia coli Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.26 58
-
 D-allose
-
 Lactococcus lactis
5.3.1.26 143
-
 D-altrose
-
 Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.26 additional information no effect of Na+, K+, Mn2+, Zn2+, Ba2+, Cu2+, Co2+, Ca2+, Mg2+, Fe3+, Al3+ Lactococcus lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.26 15000
-
 LacA subunit, SDS-PAGE Lactococcus lactis
5.3.1.26 19000
-
 LacB subunit, SDS-PAGE Lactococcus lactis
5.3.1.26 135500
-
 native enzyme by gel filtration Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.26 Lactococcus lactis P23495
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.26 of the recombinant protein by HiTrap Q HP column chromatography Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.26 1.79
-
 of the purified enzyme to produce D-allose Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.26 2 D-lyxose
-
 Lactococcus lactis D-xylulose + D-xylose
-
 r
5.3.1.26 2 D-psicose
-
 Lactococcus lactis D-allose + D-altrose
-
 r
5.3.1.26 2 L-lyxose
-
 Lactococcus lactis L-xylulose + L-xylose
-
 r
5.3.1.26 D-allose
-
 Lactococcus lactis D-psicose + D-altrose
-
 r
5.3.1.26 D-altrose
-
 Lactococcus lactis D-psicose + D-allose
-
 r
5.3.1.26 D-arabinose
-
 Lactococcus lactis D-ribulose + D-ribose
-
 r
5.3.1.26 D-fructose
-
 Lactococcus lactis D-glucose + D-mannose
-
 r
5.3.1.26 D-galactose
-
 Lactococcus lactis D-tagatose + D-talose
-
 r
5.3.1.26 D-Galactose 6-phosphate
-
 Lactococcus lactis D-Tagatose 6-phosphate
-
 r
5.3.1.26 D-glucose
-
 Lactococcus lactis D-fructose + D-mannose
-
 r
5.3.1.26 D-glucose
-
 Lactococcus lactis D-sorbose + D-idose
-
 r
5.3.1.26 D-mannose
-
 Lactococcus lactis D-fructose + D-glucose
-
 r
5.3.1.26 D-ribose
-
 Lactococcus lactis D-ribulose + D-arabinose
-
 r
5.3.1.26 D-ribulose
-
 Lactococcus lactis D-ribose + D-arabinose
-
 r
5.3.1.26 D-sorbose
-
 Lactococcus lactis D-glucose + D-idose
-
 r
5.3.1.26 D-talose
-
 Lactococcus lactis D-tagatose
-
 r
5.3.1.26 D-Xylose
-
 Lactococcus lactis D-Xylulose
-
 r
5.3.1.26 D-xylulose
-
 Lactococcus lactis D-xylose + D-lyxose
-
 r
5.3.1.26 L-fructose
-
 Lactococcus lactis L-glucose + L-mannose
-
 r
5.3.1.26 L-galactose
-
 Lactococcus lactis L-tagatose + L-talose
-
 r
5.3.1.26 L-glucose
-
 Lactococcus lactis L-fructose
-
 r
5.3.1.26 L-ribulose
-
 Lactococcus lactis L-ribose + L-arabinose
-
 r
5.3.1.26 L-tagatose
-
 Lactococcus lactis L-galactose + L-talose
-
 r
5.3.1.26 L-Xylose
-
 Lactococcus lactis L-Xylulose
-
 r
5.3.1.26 L-xylulose
-
 Lactococcus lactis L-xylose + L-lyxose
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.26 octamer hetero-octamer, LacA and LacB subunits Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
5.3.1.26 galactose-6-phosphate isomerase
-
 Lactococcus lactis
5.3.1.26 LacAB
-
 Lactococcus lactis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.26 30
-
-
 Lactococcus lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.26 7
-
-
 Lactococcus lactis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.26 9
-
-
 Lactococcus lactis