Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Schwarz, A.; Goedl, C.; Minani, A.; Nidetzky, B.
    Trehalose phosphorylase from Pleurotus ostreatus: characterization and stabilization by covalent modification, and application for the synthesis of alpha,alpha-trehalose (2007), J. Biotechnol., 129, 140-150.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.231 synthesis sucrose is converted to alpha,alpha-trehalose in about 60% yield using a coupled enzyme system composed of sucrose phosphorylase from Leuconostoc mesenteroides, glucose isomerase from Streptomyces murinus and the appropriately stabilized PoTPase Pleurotus ostreatus

General Stability

EC Number General Stability Organism
2.4.1.231 unstable protein, half-life of no more than approximately 1.3 h in 20mM MES buffer, 25°C, pH 7.0. The enzyme can be stabilized on addition of alpha,alpha-trehalose (300 mM) which yields a half-life of 11.5 h. In the presence of 20% (w/v) glycerol, and 9 and 26% (w/v) PEG-4000, half-life is 6.5 h, 16 h and 70 h, respectively. Large improvements in stability by covalent protein modification with activated derivatives of PEG-5000 Pleurotus ostreatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.231 vanadate
-
 Pleurotus ostreatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.231 3.5
-
 phosphate
-
 Pleurotus ostreatus
2.4.1.231 3.5
-
 phosphate 30°C, pH 6.8 Pleurotus ostreatus
2.4.1.231 4.1
-
 alpha-D-glucose 1-phosphate
-
 Pleurotus ostreatus
2.4.1.231 4.1
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.8 Pleurotus ostreatus
2.4.1.231 40
-
 alpha-D-glucose
-
 Pleurotus ostreatus
2.4.1.231 40
-
 alpha-D-glucose 30°C, pH 6.8 Pleurotus ostreatus
2.4.1.231 79
-
 alpha,alpha-trehalose
-
 Pleurotus ostreatus
2.4.1.231 79
-
 alpha,alpha-trehalose 30°C, pH 6.8 Pleurotus ostreatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.231 55000
-
 SDS PAGE Pleurotus ostreatus
2.4.1.231 58300
-
 gel filtration Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.231 Pleurotus ostreatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.231
-
 Pleurotus ostreatus
2.4.1.231 trehalose phosphorylase from the basidiomycete Pleurotus ostreatus (PoTPase) is isolated from fungal fruit bodies through 500-fold purification with a yield of 44% Pleurotus ostreatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.231 fruit body
-
 Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.231 additional information
-
-
 Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.231 2-dehydro-D-glucose + alpha-D-glucose 1-phosphate
-
 Pleurotus ostreatus ? + phosphate
-
 ?
2.4.1.231 2-deoxy-D-glucose + alpha-D-glucose 1-phosphate 8% of the activity with alpha-D-glucose Pleurotus ostreatus ?
-
 r
2.4.1.231 2-deoxy-D-glucose + alpha-D-glucose 1-phosphate
-
 Pleurotus ostreatus ? + phosphate
-
 ?
2.4.1.231 2-fluoro-D-glucose + alpha-D-glucose 1-phosphate 15% of the activity with alpha-D-glucose Pleurotus ostreatus ?
-
 r
2.4.1.231 2-fluoro-D-glucose + alpha-D-glucose 1-phosphate
-
 Pleurotus ostreatus ? + phosphate
-
 ?
2.4.1.231 2-keto-D-glucose + alpha-D-glucose 1-phosphate 50% of the activity with alpha-D-glucose Pleurotus ostreatus ?
-
 r
2.4.1.231 alpha,alpha-trehalose + arsenate 30% of the activity with phosphate Pleurotus ostreatus alpha-D-glucose 1-arsenate + alpha-D-glucose
-
 ?
2.4.1.231 alpha,alpha-trehalose + arsenate
-
 Pleurotus ostreatus alpha-D-glucose + alpha-D-glucose 1-arsenate
-
 r
2.4.1.231 alpha,alpha-trehalose + phosphate
-
 Pleurotus ostreatus alpha-D-glucose + alpha-D-glucose 1-phosphate
-
 r
2.4.1.231 alpha-D-glucose + alpha-D-glucose 1-phosphate beta-D-glucose 1-phosphate is not turned over by the enzyme Pleurotus ostreatus alpha,alpha-trehalose + phosphate
-
 r
2.4.1.231 D-mannose + alpha-D-glucose 1-phosphate 3% of the activity with alpha-D-glucose Pleurotus ostreatus ?
-
 r
2.4.1.231 D-mannose + alpha-D-glucose 1-phosphate
-
 Pleurotus ostreatus ? + phosphate
-
 ?
2.4.1.231 additional information no measurable activity is found with D-arabinose, D-fructose, D-fucose, D-galactose, D-lyxose, D-mannoheptaose, D-ribose and D-xylose Pleurotus ostreatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.231 monomer 1 * 55000, SDS-PAGE Pleurotus ostreatus
2.4.1.231 monomer gel filtration Pleurotus ostreatus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.231 PoTPase
-
 Pleurotus ostreatus
2.4.1.231 trehalose phosphorylase
-
 Pleurotus ostreatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.231 30
-
 assay at Pleurotus ostreatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.231 additional information
-
 PoTPase has a half life of approximately 1 h at 30°C Pleurotus ostreatus
2.4.1.231 30
-
 half-life: approximately 1 h in absence of stabilizing compound, 11.5 h in presence of 300 mM alpha,alpha-trehalose, 6.5 h in presence of 20% w/v glycerol, 70 h in presence of 26% w/v polyethylenglycol. Covalent modification of PoTPase with activated derivatives of PEG 5000 increases the stability by up to 600fold Pleurotus ostreatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.231 0.27
-
 2-deoxy-D-glucose
-
 Pleurotus ostreatus
2.4.1.231 0.42
-
 D-mannose
-
 Pleurotus ostreatus
2.4.1.231 0.89
-
 2-fluoro-D-glucose
-
 Pleurotus ostreatus
2.4.1.231 1.15
-
 arsenate
-
 Pleurotus ostreatus
2.4.1.231 4.5
-
 2-dehydro-D-glucose
-
 Pleurotus ostreatus
2.4.1.231 16
-
 alpha-D-glucose 1-phosphate
-
 Pleurotus ostreatus
2.4.1.231 16
-
 alpha-D-glucose 1-phosphate 30°C, pH 6.8 Pleurotus ostreatus
2.4.1.231 16.8
-
 alpha-D-glucose
-
 Pleurotus ostreatus
2.4.1.231 16.8
-
 alpha-D-glucose 30°C, pH 6.8 Pleurotus ostreatus
2.4.1.231 17.6
-
 phosphate
-
 Pleurotus ostreatus
2.4.1.231 17.6
-
 phosphate 30°C, pH 6.8 Pleurotus ostreatus
2.4.1.231 18.3
-
 alpha,alpha-trehalose
-
 Pleurotus ostreatus
2.4.1.231 18.3
-
 alpha,alpha-trehalose 30°C, pH 6.8 Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.231 5.8 6.4 synthesis of alpha,alpha-trehalose Pleurotus ostreatus
2.4.1.231 6.2
-
 alpha, alpha-trehalose synthesis Pleurotus ostreatus
2.4.1.231 6.8
-
 assay at Pleurotus ostreatus
2.4.1.231 6.8
-
 alpha, alpha-trehalose phosphorolysis Pleurotus ostreatus
2.4.1.231 6.8 7.2 phosphorolysis Pleurotus ostreatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.231 5.5 7.8 pH 5.5: about 75% of maximal activity, pH 7.8: about 80% of maxuimal activity, phosphorolysis Pleurotus ostreatus
2.4.1.231 5.5 6.8 pH 5.5: about 80% of maximal activity, pH 75% of maximal activity, synthesis of alpha,alpha-trehalose Pleurotus ostreatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.231 0.004
-
 vanadate
-
 Pleurotus ostreatus