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Literature summary extracted from

  • Okochi, M.; Nakagawa, I.; Kobayashi, T.; Hayashi, S.; Furusaki, S.; Honda, H.
    Enhanced activity of 3alpha-hydroxysteroid dehydrogenase by addition of the co-solvent 1-butyl-3-methylimidazolium (l)-lactate in aqueous phase of biphasic systems for reductive production of steroids (2006), J. Biotechnol., 128, 376-382.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.50 1-butyl-3-methylimidazolium L-lactate activates the enzyme as 5% co-solvent in Tris-HCl buffer, overview Comamonas testosteroni
1.1.1.50 1-butyl-3-methylimidazolium L-lactate enhanced activity of 3alpha-hydroxysteroid dehydrogenase by addition of the co-solvent to 50 mM Tris-HCl buffer in aqueous phase of biphasic systems for reductive production of steroids. In a coupled-enzyme system comprising HSDH and formate dehydrogenase, a twofold increase in production rate of androsterone is obtained when utilizing 1-butyl-3-methylimidazolium L-lactate with NADH regeneration, overview Comamonas testosteroni
1.1.1.50 1-ethyl-3-methylimidazolium trifluoromethanesulfonate addition of the co-solvent leads to slightly enhanced enzyme activity n an aqueous-organic solvent system with Tris-HCl buffer, overview Comamonas testosteroni
1.1.1.50 additional information poor activation by 1-butyl-3-methylimidazolium trifluoromethanesulfonate and 1-butyl-3-methylimidazolium tetrafluoroborate Comamonas testosteroni
1.1.1.213 1-butyl-3-methylimidazolium L-lactate enhanced activity of 3alpha-hydroxysteroid dehydrogenase by addition of the co-solvent to 50 mM Tris-HCl buffer in aqueous phase of biphasic systems for reductive production of steroids. In a coupled-enzyme system comprising HSDH and formate dehydrogenase, a twofold increase in production rate of androsterone is obtained when utilizing 1-butyl-3-methylimidazolium L-lactate with NADH regeneration, overview Comamonas testosteroni
1.1.1.213 1-ethyl-3-methylimidazolium trifluoromethanesulfonate addition of the co-solvent leads to slightly enhanced enzyme activity n an aqueous-organic solvent system with Tris-HCl buffer, overview Comamonas testosteroni
1.1.1.213 additional information poor activation by 1-butyl-3-methylimidazolium trifluoromethanesulfonate and 1-butyl-3-methylimidazolium tetrafluoroborate Comamonas testosteroni

Application

EC Number Application Comment Organism
1.1.1.50 synthesis the enzyme is useful foe androsterone production in a coupled system with formate dehydrogenase in enhancing Tris-HCl/co-solvent 1-butyl-3-methylimidazolium L-lactate, at pH 7.6 and 25°C, method optimization, overview Comamonas testosteroni
1.1.1.50 synthesis the enzyme is useful in reductive production of steroids. In a coupled-enzyme system comprising HSDH and formate dehydrogenase, a twofold increase in production rate of androsterone is obtained when utilizing 1-butyl-3-methylimidazolium L-lactate with NADH regeneration Comamonas testosteroni
1.1.1.213 synthesis the enzyme is useful in reductive production of steroids. In a coupled-enzyme system comprising HSDH and formate dehydrogenase, a twofold increase in production rate of androsterone is obtained when utilizing 1-butyl-3-methylimidazolium L-lactate with NADH regeneration Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 additional information
-
 additional information kinetics in absence and presence of co-solvents, overview Comamonas testosteroni
1.1.1.213 additional information
-
 additional information kinetics in absence and presence of co-solvents, overview Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 androsterone + NAD+ Comamonas testosteroni
-
 5alpha-androstane-3,17-dione + NADH
-
 r
1.1.1.213 androsterone + NAD+ Comamonas testosteroni
-
 5alpha-androstane-3,17-dione + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Comamonas testosteroni
-
-
-
1.1.1.213 Comamonas testosteroni
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.50 commercial preparation
-
 Comamonas testosteroni
-
1.1.1.213 commercial preparation
-
 Comamonas testosteroni
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.50 7
-
 commercially purified enzyme in Tris-HCl/co-solvent 1-butyl-3-methylimidazolium L-lactate at pH 7.6 and 25°C Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 androsterone + NAD+
-
 Comamonas testosteroni 5alpha-androstane-3,17-dione + NADH
-
 r
1.1.1.213 androsterone + NAD+
-
 Comamonas testosteroni 5alpha-androstane-3,17-dione + NADH
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 3alpha-hydroxysteroid dehydrogenase
-
 Comamonas testosteroni
1.1.1.50 HSDH
-
 Comamonas testosteroni
1.1.1.50 More cf. EC 1.1.1.213 Comamonas testosteroni
1.1.1.213 HSDH
-
 Comamonas testosteroni
1.1.1.213 More cf. EC 1.1.1.50 Comamonas testosteroni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.50 25
-
 assay at Comamonas testosteroni
1.1.1.213 25
-
 assay at Comamonas testosteroni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 8.2
-
 assay at Comamonas testosteroni

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NAD+
-
 Comamonas testosteroni
1.1.1.50 NADH
-
 Comamonas testosteroni
1.1.1.213 NAD+
-
 Comamonas testosteroni
1.1.1.213 NADH
-
 Comamonas testosteroni