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Literature summary extracted from

  • Stockman, B.J.; Lodovice, I.J.; Fisher, D.A.; McColl, A.S.; Xie, Z.
    A nuclear magnetic resonance-based functional assay for nicotinamide adenine dinucleotide synthetase (2007), J. Biomol. Screen., 12, 457-463.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.5 expressed in Escherichia coli BL21 (DE3) cells Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.5 0.03
-
 deamido-NAD+ in 60 mM Tris-HCl pH 8.0, 10 mM NH4Cl, 10 mM MgCl2, 10 mM KCl Staphylococcus aureus
6.3.1.5 0.2
-
 ATP in 60 mM Tris-HCl pH 8.0, 10 mM NH4Cl, 10 mM MgCl2, 10 mM KCl Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.5 Staphylococcus aureus
-
 strain 8325-4
-
6.3.1.5 Staphylococcus aureus NCTC 8325
-
 strain 8325-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.5 Ni-NTA Superflow resin chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Staphylococcus aureus AMP + diphosphate + NAD+
-
 ?
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Staphylococcus aureus NCTC 8325 AMP + diphosphate + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.5 NAD synthetase
-
 Staphylococcus aureus
6.3.1.5 NadE
-
 Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.5 ATP
-
 Staphylococcus aureus