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Literature summary extracted from

  • van der Linden, E.; Burgdorf, T.; de Lacey, A.L.; Buhrke, T.; Scholte, M.; Fernandez, V.M.; Friedrich, B.; Albracht, S.P.
    An improved purification procedure for the soluble [NiFe]-hydrogenase of Ralstonia eutropha: new insights into its (in)stability and spectroscopic properties (2006), J. Biol. Inorg. Chem., 11, 247-260.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.1.2 soluble
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 Cupriavidus necator
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Organism

EC Number Organism UniProt Comment Textmining
1.12.1.2 Cupriavidus necator
-
-
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1.12.1.2 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
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-
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Oxidation Stability

EC Number Oxidation Stability Organism
1.12.1.2 air the presence of NADH leads to rapid destruction of the hexameric enzyme Cupriavidus necator
1.12.1.2 the hexameric SH preparation is both active and stable in the presence of NADPH and air Cupriavidus necator

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.1.2 improved purification procedure Cupriavidus necator

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.12.1.2 additional information cells are grown heterotrophically in a fructose-glycerol minimal medium Cupriavidus necator
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.1.2 additional information
-
-
 Cupriavidus necator

Synonyms

EC Number Synonyms Comment Organism
1.12.1.2 HoxFUYHI2 hexameric enzyme form Cupriavidus necator
1.12.1.2 NAD+-reducing [NiFe]-hydrogenase
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 Cupriavidus necator