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Literature summary extracted from

  • Gray, J.P.; Heck, D.E.; Mishin, V.; Smith, P.J.; Hong, J.Y.; Thiruchelvam, M.; Cory-Slechta, D.A.; Laskin, D.L.; Laskin, J.D.
    Paraquat increases cyanide-insensitive respiration in murine lung epithelial cells by activating an NAD(P)H: paraquat oxidoreductase: Identification of the enzyme as thioredoxin reductase (2007), J. Biol. Chem., 282, 7939-7949.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.9 1-chloro-2,4-dinitrobenzene 0.1 mM Mus musculus
1.8.1.9 diphenylene iodonium IC50: 0.001 mM Mus musculus
1.8.1.9 sodium aurothiomalate 0.1 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 additional information
-
 additional information effects of paraquat on reaction kinetics of NADH and NADPH in lung cell extracts Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.9 cytoplasm
-
 Mus musculus 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 58000
-
 SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.9 additional information Mus musculus the enzyme can promote oxidative stress by redox cycling of paraquat: paraquat + O2 + NADPH + H+ --> paraquat radical + O2- radical + NADP+ ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9
-
 Mus musculus
1.8.1.9 2',5'-ADP-agarose affinity chromatography and Superose size exclusion chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.9 lung epithelium
-
 Mus musculus
-
1.8.1.9 lung epithelium MLE-15 cell line Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
 Mus musculus 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
-
 Mus musculus 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 additional information the enzyme can promote oxidative stress by redox cycling of paraquat: paraquat + O2 + NADPH + H+ --> paraquat radical + O2- radical + NADP+ Mus musculus ?
-
 ?
1.8.1.9 paraquat + NAD(P)H
-
 Mus musculus paraquat radical + NAD(P)+
-
 r
1.8.1.9 paraquat radical + O2
-
 Mus musculus paraquat + O2-
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 NAD(P)H:paraquat oxidoreductase
-
 Mus musculus
1.8.1.9 thioredoxin reductase
-
 Mus musculus
1.8.1.9 TrxR
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
 Mus musculus
1.8.1.9 NADH
-
 Mus musculus
1.8.1.9 NADPH
-
 Mus musculus
1.8.1.9 NADPH approximately four times more activity was evident with NADPH when compared to NADH Mus musculus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.8.1.9 0.001
-
 IC50: 0.001 mM Mus musculus diphenylene iodonium