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Literature summary extracted from

  • Gong, H.; Murphy, A.; McMaster, C.R.; Byers, D.M.
    Neutralization of acidic residues in helix II stabilizes the folded conformation of acyl carrier protein and variably alters its function with different enzymes (2007), J. Biol. Chem., 282, 4494-4503.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.129 myristoyl-[acyl-carrier protein]
-
 Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.129 additional information
-
 additional information the kinetic analysis of LpxA is complicated by the thermodynamically unfavorable reaction in the forward direction, overview Vibrio harveyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.129 Ca2+ not as effective as Mg2+ Vibrio harveyi
2.3.1.129 Mg2+ is required for stabilization of substrate acyl-carrier protein in a helical conformation Vibrio harveyi
2.3.1.129 Mn2+ not as effective as Mg2+ Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine Vibrio harveyi
-
 [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.129 Vibrio harveyi
-
 gene lpxA
-
2.7.8.7 Vibrio harveyi
-
-
-
6.2.1.20 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.20 partial purification by DEAE-Sepharose chromatography and Sephacryl S-300 gel filtration Vibrio harveyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.20 0.00000478
-
 at 37°C for 30 min with 10 mM MgSO4, 10 mM ATP, 0.1 mM [9,10-3H]myristic acid in 100 mM Tris-HCl, pH 7.8, and 5 mM dithiothreitol Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Vibrio harveyi [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine i.e. (R)-3-hydroxymyristoyl-[acyl-carrier protein], the forward reaction direction is thermodynamically unfavorable, recombinant His-tagged enzyme, the activity with mutant acyl-carrier proteins is impaired, especially with mutations of the B-site of the acyl-carrier protein, recombinant wild-type and mutant ACP substrates, overview Vibrio harveyi [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
2.7.8.7 additional information D35 in site A of acyl carrier protein is critical for enzyme activity Vibrio harveyi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.129 LpxA
-
 Vibrio harveyi
2.3.1.129 UDP-N-acetylglucosamine acyltransferase
-
 Vibrio harveyi
6.2.1.20 AAS
-
 Vibrio harveyi
6.2.1.20 AcpS
-
 Vibrio harveyi
6.2.1.20 Acyl-ACP synthetase
-
 Vibrio harveyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.129 25
-
 assay at Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.129 8
-
 assay at Vibrio harveyi