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Literature summary extracted from

  • Jerga, A.; Lu, Y.J.; Schujman, G.E.; de Mendoza, D.; Rock, C.O.
    Identification of a soluble diacylglycerol kinase required for lipoteichoic acid production in Bacillus subtilis (2007), J. Biol. Chem., 282, 21738-21745.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.107 expression as His-tagged protein, Escherichia coli Bacillus subtilis
2.7.1.107 expression in Escherichia coli Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.107 additional information functional complementation of Escherichia coli dgkA mutant. Conditional inactivation of gene expression leads to the accumulation of diacylglycerol and the cessation of lipoteichoic acid formation in Bacillus subtilis Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.107 soluble
-
 Bacillus subtilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.107 34000
-
 x * 34000, calculated and SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.107 additional information Bacillus subtilis the soluble diacylglycerol kinase DgkB is required for lipoteichoic acid production in Bacillus subtilis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.107 Bacillus subtilis
-
-
-
2.7.1.107 Bacillus subtilis P19638
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.107 recombinant protein Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.107 4.8
-
 substrate 1,2-dioctanoyl-sn-glycerol, pH 7.0, 25°C Bacillus subtilis
2.7.1.107 89
-
 substrate 1,2-dioleoyl-sn-glycerol, pH 7.0, 25°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.107 ATP + 1,2-diacyl-sn-glycerol
-
 Bacillus subtilis ADP + 1,2-diacyl-sn-glycerol 3-phosphate enzyme is more active toward long-chain diacylglycerol compared with short-chain diacylglycerol ?
2.7.1.107 ATP + 1,2-dioctanoyl-sn-glycerol
-
 Bacillus subtilis ADP + 1,2-dioctanoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 ATP + 1,2-dioleoyl-sn-glycerol
-
 Bacillus subtilis ADP + 1,2-dioleoyl-sn-glycerol 3-phosphate
-
 ?
2.7.1.107 additional information the soluble diacylglycerol kinase DgkB is required for lipoteichoic acid production in Bacillus subtilis Bacillus subtilis ?
-
 ?
2.7.1.107 additional information no substrate: monoacylglycerol, ceramide, or undecaprenol Bacillus subtilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.107 ? x * 34000, calculated and SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.107 DgkB
-
 Bacillus subtilis
2.7.1.107 YerQ
-
 Bacillus subtilis