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Literature summary extracted from

  • Maruyama, D.; Nishitani, Y.; Nonaka, T.; Kita, A.; Fukami, T.A.; Mio, T.; Yamada-Okabe, H.; Yamada-Okabe, T.; Miki, K.
    Crystal structure of uridine-diphospho-N-acetylglucosamine pyrophosphorylase from Candida albicans and catalytic reaction mechanism (2007), J. Biol. Chem., 282, 17221-17230.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.23 expressed in Escherichia coli JM109 cells as a glutathione S-transferase fusion protein Candida albicans

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.23 hanging drop vapour diffusion method Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.23 cytoplasm
-
 Candida albicans 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.23 Ca2+
-
 Candida albicans
2.7.7.23 Ca2+ Ca2+ shows similar enhancement like Mg2+ Candida albicans
2.7.7.23 Mg2+
-
 Candida albicans
2.7.7.23 Mg2+ Mg2+ enhances the enzymatic activity by increasing the affinity between UTP and the enzyme Candida albicans
2.7.7.23 Zn2+
-
 Candida albicans
2.7.7.23 Zn2+ Zn2+ shows similar enhancement like Mg2+ Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.23 54700
-
-
 Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.23 N-acetylglucosamine 1-phosphate + uridine 5'-triphosphate Candida albicans
-
 uridine-diphospho-N-acetylglucosamine + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.23 Candida albicans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.23 glutathione Sepharose column chromatography and Superdex 75 gel filtration Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.23 diphosphate + UDP-N-acetyl-D-glucosamine the reaction proceeds as an SN2 reaction Candida albicans UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
2.7.7.23 N-acetylglucosamine 1-phosphate + uridine 5'-triphosphate
-
 Candida albicans uridine-diphospho-N-acetylglucosamine + diphosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.7.23 GlmU
-
 Candida albicans
2.7.7.23 UAP enzyme
-
 Candida albicans
2.7.7.23 UAP1
-
 Candida albicans
2.7.7.23 UDP-GlcNAc pyrophosphorylase
-
 Candida albicans
2.7.7.23 uridine-diphospho-N-acetylglucosamine pyrophosphorylase
-
 Candida albicans