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Literature summary extracted from

  • Schauble, S.; King, C.C.; Darshi, M.; Koller, A.; Shah, K.; Taylor, S.S.
    Identification of ChChd3 as a novel substrate of the cAMP-dependent protein kinase (PKA) using an analog-sensitive catalytic subunit (2007), J. Biol. Chem., 282, 14952-14959.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.11 cAMP
-
 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.11 functional expression of His-tagged mutant catalytic subunit M120G in Escherichia coli strain BL21(DE3) requiring co-expression with PDK1 for stabilization, overview Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.11 M120G site-directed mutagenesis in the ATP-binding pocket of the catalytic subunit, engineering of the PKA catalytic subunit to accept bulky N6-substituted ATP analogs, using a chemical genetics approach initially pioneered with v-Src, overview, N6(benzyl)-ATP and N6(phenethyl)-ATP are the prefrred cofactors of the mutant Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.11 N6-benzyl-ATP preferred co-substrate of catalytic subunit mutant M120G, but inhibitory for the mutant, not the wild-type enzyme, versus kemptide, overview Mus musculus
2.7.11.11 N6-phenethyl-ATP preferred co-substrate of catalytic subunit mutant M120G, but inhibitory for the mutant, not the wild-type enzyme, versus kemptide, overview Mus musculus
2.7.11.11 regulatory subunit 1alpha
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.11 0.0011
-
 N6-benzyl-ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
2.7.11.11 0.0015
-
 N6-phenethyl-ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
2.7.11.11 0.0174
-
 ATP pH 7.0, 37°C, recombinant wild-type catalytic subunit Mus musculus
2.7.11.11 0.0305
-
 ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
2.7.11.11 0.1
-
 N6-benzyl-ATP above, pH 7.0, 37°C, recombinant wild-type catalytic subunit Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.11 mitochondrion
-
 Mus musculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+
-
 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.11 phosphoprotein the catalytically active subunit mutant M120G is phosphorylated on Thr197 and Ser338 Mus musculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 recombinant His-tagged mutant catalytic subunit M120G from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.11 brain
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.11 additional information
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.11 ATP + ChChd3 an endogenous mitochondrial protein Mus musculus ADP + phosphorylated ChChd3
-
 ?
2.7.11.11 ATP + kemptide
-
 Mus musculus ADP + phospho-kemptide
-
 ?
2.7.11.11 N6-benzyl-ATP + ChChd3 cofactor of mutant M120G, poor activity with the wild-type catalytic subunit Mus musculus N6-benzyl-ADP + phosphorylated ChChd3
-
 ?
2.7.11.11 N6-phenethyl-ATP + ChChd3 cofactor of mutant M120G, poor activity with the wild-type catalytic subunit Mus musculus N6-phenethyl-ADP + phosphorylated ChChd3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.11 37
-
 assay at Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.11 0.12
-
 N6-phenethyl-ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
2.7.11.11 0.5
-
 N6-benzyl-ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
2.7.11.11 10.6
-
 ATP pH 7.0, 37°C, recombinant mutant M120G catalytic subunit Mus musculus
2.7.11.11 19.6
-
 ATP pH 7.0, 37°C, recombinant wild-type catalytic subunit Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.11 7
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.11 ATP preferred cofactor of the wild-type catalytic subunit Mus musculus
2.7.11.11 N6-benzyl-ATP preferred co-substrate of catalytic subunit mutant M120G, but inhibitory versus kemptide, overview Mus musculus
2.7.11.11 N6-phenethyl-ATP preferred co-substrate of catalytic subunit mutant M120G, but inhibitory versus kemptide, overview Mus musculus