EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.2.B14 | expressed in Escherichia coli | Bacillus subtilis |
2.3.2.B14 | expressed in Escherichia coli | Enterococcus faecalis |
2.3.2.B14 | expressed in Escherichia coli | Enterococcus faecium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.2.B14 | Bacillus subtilis | - |
- |
- |
2.3.2.B14 | Bacillus subtilis 168 | - |
- |
- |
2.3.2.B14 | Enterococcus faecalis | - |
- |
- |
2.3.2.B14 | Enterococcus faecalis JH2-2 | - |
- |
- |
2.3.2.B14 | Enterococcus faecium | - |
- |
- |
2.3.2.B14 | Enterococcus faecium M512 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.2.B14 | affinity anion exchange chromatography and gel filtration | Enterococcus faecium |
2.3.2.B14 | Ni2+ nitrilotriacetate-agarose resin affinity chromatography | Bacillus subtilis |
2.3.2.B14 | Ni2+ nitrilotriacetate-agarose resin affinity chromatography, MonoQ HR5/5 chromatography, and Superdex HR10/30 column gel filtration | Enterococcus faecalis |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.2.B14 | -20°C, 50 mM Tris-HCl pH 7.5 containing 100 mM NaCl | Bacillus subtilis |
2.3.2.B14 | -80 °C, 50 mM Tris-HCl pH 7.5 containing 300 mM NaCl | Enterococcus faecalis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.2.B14 | (L-Lys)3-D-iso-Asn + (L-Lys)3-L-Ala-L-Ala | - |
Enterococcus faecium | ? | - |
? | |
2.3.2.B14 | (L-Lys)3-D-iso-Asn + (L-Lys)3-L-Ala-L-Ala | - |
Enterococcus faecium M512 | ? | - |
? | |
2.3.2.B14 | (L-Lys)3-L-Ala-L-Ala + ? | - |
Bacillus subtilis | ? | - |
? | |
2.3.2.B14 | (L-Lys)3-L-Ala-L-Ala + ? | - |
Bacillus subtilis 168 | ? | - |
? | |
2.3.2.B14 | meso-diaminopimelic acid + (L-Lys)3-D-iso-Asn | - |
Enterococcus faecalis | ? | - |
? | |
2.3.2.B14 | meso-diaminopimelic acid + (L-Lys)3-D-iso-Asn | - |
Enterococcus faecalis JH2-2 | ? | - |
? | |
2.3.2.B14 | meso-diaminopimelic acid + (L-Lys)3-L-Ala-L-Ala | - |
Enterococcus faecalis | ? | - |
? | |
2.3.2.B14 | meso-diaminopimelic acid + (L-Lys)3-L-Ala-L-Ala | - |
Enterococcus faecalis JH2-2 | ? | - |
? | |
2.3.2.B14 | additional information | the enzyme is specific for disaccharide-peptides containing an L-Ala-L-Ala side chain in both the acceptor and donor substrates | Bacillus subtilis | ? | - |
? | |
2.3.2.B14 | additional information | the enzyme is specific for disaccharide-peptides containing an L-Ala-L-Ala side chain in both the acceptor and donor substrates | Bacillus subtilis 168 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.2.B14 | L,D-transpeptidase | - |
Bacillus subtilis |
2.3.2.B14 | L,D-transpeptidase | - |
Enterococcus faecalis |
2.3.2.B14 | L,D-transpeptidase | - |
Enterococcus faecium |
2.3.2.B14 | LdtBS | - |
Bacillus subtilis |
2.3.2.B14 | Ldtfm | - |
Enterococcus faecium |
2.3.2.B14 | Ldtfs | - |
Enterococcus faecalis |