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Literature summary extracted from

  • Hrmova, M.; Farkas, V.; Lahnstein, J.; Fincher, G.B.
    A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans (2007), J. Biol. Chem., 282, 12951-12962.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.207 Mg2+ inhibits activity by 3-4% at 5-15 mM Hordeum vulgare

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.207 Ca2+ stimulates activity by 7-8% at 5-15 mM Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.207 31000
-
 SDS-PAGE Hordeum vulgare

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.4.1.207 Glycerol the addition of 10% (v/v) glycerol has no affect on XET activity after several freeze-thawing cycles Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Hordeum vulgare
-
 cultivar Clipper
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207 ammonium sulfate precipitation, Sepharose Q column chromatography, phenyl Sepharose column chromatography, chromatofocusing on PBE-94, and Bio-Gel P-60 gel filtration Hordeum vulgare
2.4.1.207 Sepharose Q column chromatography, Phenyl-Sepharose chromatography, PBE-94 column chromatography and Bio-Gel P-60 gel filtration Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 seedling
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.207 0.000000072
-
 enzyme from crude homogenate, at 30°C Hordeum vulgare
2.4.1.207 0.0002178
-
 after 3025fold purification, at 30°C Hordeum vulgare
2.4.1.207 0.02
-
 crude cell extract, at 30°C in 100 mM succinate or ammonium acetate buffers, pH 6.0, containing 5 mM calcium chloride Hordeum vulgare
2.4.1.207 60.51
-
 after 3025fold purification, at 30°C in 100 mM succinate or ammonium acetate buffers, pH 6.0, containing 5 mM calcium chloride Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
2.4.1.207 -20°C, 100 mM succinate or ammonium acetate buffers at pH 6.0 containing 5 mM calcium chloride, 1 year, no loss of activity Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor barley (1,3,1,4)-beta-D-glucan + acceptor cellooligosaccharide 0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide Hordeum vulgare ?
-
 ?
2.4.1.207 donor barley (1,3,1,4)-beta-D-glucan + acceptor xyloglucan oligosaccharide 0.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide Hordeum vulgare ?
-
 ?
2.4.1.207 donor carboxymethylcellulose + acceptor xyloglucan oligosaccharide 0.4% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide Hordeum vulgare ?
-
 ?
2.4.1.207 donor hydroxyethylcellulose + acceptor cellooligosaccharide 0.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide Hordeum vulgare ?
-
 ?
2.4.1.207 donor hydroxyethylcellulose + acceptor xyloglucan oligosaccharide 44.2% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide Hordeum vulgare ?
-
 ?
2.4.1.207 donor locust bean gum + acceptor xyloglucan oligosaccharide 0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide Hordeum vulgare ?
-
 ?
2.4.1.207 donor sulfuric acid-swollen cellulose + acceptor xyloglucan oligosaccharide 5% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide Hordeum vulgare ?
-
 ?
2.4.1.207 donor tamarind xyloglucan + acceptor cellooligosaccharide 0.1% relative activity compared to the reaction with donor tamarind xyloglucan and acceptor xyloglucan oligosaccharide Hordeum vulgare ?
-
 ?
2.4.1.207 donor tamarind xyloglucan + acceptor xyloglucan oligosaccharide
-
 Hordeum vulgare ?
-
 ?
2.4.1.207 additional information no transferase activities are detected with laminarin, lichenin, pustulan, barley arabinoxylan, Konjac glucomannan, citrus fruit pectin, and esterified pectin (potassium salt), orange polygalactouronic acid (sodium salt), soybean pectic fiber rhamnogalactouronan, beta-D-galactans, and arabinogalactan protein with xyloglucan oligosaccharide and cellooligosaccharide, no transferase activity is detected laminari-oligosaccharides as acceptor substrates Hordeum vulgare ?
-
 ?
2.4.1.207 additional information no transferase activity is detected with laminarin, lichenin, pustulan, barley arabinoxylan, glucomannan, citrus fruit pectin or esterified pectin (potassium salt), orange polygalacturonic acid (sodium salt), soybean pectic fiber rhamnogalactouronan, lupin or potato beta-D-galactans, or gum arabic Hordeum vulgare ?
-
 ?
2.4.1.207 additional information XET5 catalyzes the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates and between xyloglucans and (1,3 1,4)-beta-D-glucans Hordeum vulgare ?
-
 ?
2.4.1.207 xyloglucan + barley (1,3 1,4)-beta-D-glucan the rate of covalent bond formation with barley (1,3 1,4)-beta-D-glucan is significant but slower compared with that on tamarind xyloglucan Hordeum vulgare ?
-
 ?
2.4.1.207 xyloglucan + heptaitol
-
 Hordeum vulgare ?
-
 ?
2.4.1.207 xyloglucan + hydroxyethylcellulose the rate of covalent bond formation with hyrdoxyethylcellulose is comparable with that on tamarind xyloglucan Hordeum vulgare ?
-
 ?
2.4.1.207 xyloglucan + sulfuric acid-swollen cellulose
-
 Hordeum vulgare ?
-
 ?
2.4.1.207 xyloglucan + tamarind xyloglucan
-
 Hordeum vulgare ?
-
 ?
2.4.1.207 xyloglucan + Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
-
 Hordeum vulgare ?
-
 ?
2.4.1.207 xyloglucan + Xylalpha(1,6)Glc-Xylalpha(1,6)Glc-Galalpha(1,2)Xylalpha(1,6)Glc-Glc
-
 Hordeum vulgare ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.207 XET5
-
 Hordeum vulgare
2.4.1.207 xyloglucan endo-transglucosylase
-
 Hordeum vulgare
2.4.1.207 xyloglucan xyloglucosyl transferase
-
 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.207 28 30
-
 Hordeum vulgare

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.207
-
-
 the enzyme also operates efficiently at 0 °C, where it shows almost 40% of the activity observed at 30°C Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.207 6
-
-
 Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.207 Hordeum vulgare isoelectric focusing
-
 8.5

General Information

EC Number General Information Comment Organism
2.4.1.207 physiological function xyloglucan endo-transglucosylase can link different polysaccharides in vivo and hence influence cell wall strength, flexibility, and porosity Hordeum vulgare