BRENDA - Enzyme Database

The Helicobacter pylori amidotransferase GatCAB is equally efficient in glutamine-dependent transamidation of Asp-tRNAAsn and Glu-tRNAGln

Sheppard, K.; Akochy, P.M.; Salazar, J.C.; Soell, D.; J. Biol. Chem. 282, 11866-11873 (2007)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
6.3.5.6
S128T
mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln
Helicobacter pylori
6.3.5.6
S152A
mutant is glutaminase inactive
Helicobacter pylori
6.3.5.6
S152T
mutant is glutaminase inactive
Helicobacter pylori
6.3.5.7
S128T
mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln
Helicobacter pylori
6.3.5.7
S152A
mutant is glutaminase inactive
Helicobacter pylori
6.3.5.7
S152T
mutant is glutaminase inactive
Helicobacter pylori
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.6
0.00095
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.00118
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.0207
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.0224
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.0402
-
Gln
37C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
6.3.5.6
0.0509
-
Gln
37C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity
Helicobacter pylori
6.3.5.6
0.2068
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.00095
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.00118
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.0207
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.0224
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.0402
-
Gln
37C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
6.3.5.7
0.0509
-
Gln
37C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity
Helicobacter pylori
6.3.5.7
0.2068
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.3.5.6
Helicobacter pylori
-
-
-
6.3.5.7
Helicobacter pylori
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.5.6
-
Helicobacter pylori
6.3.5.7
-
Helicobacter pylori
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.6
ATP + Asp-tRNAAsn + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency
674787
Helicobacter pylori
ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
-
-
?
6.3.5.6
ATP + Glu-tRNAGln + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine
674787
Helicobacter pylori
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + Asp-tRNAAsn + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency
674787
Helicobacter pylori
ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine
674787
Helicobacter pylori
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.5.6
0.027
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.052
2.1
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
6.3.5.6
1.3
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
3
6
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
3.49
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
3.61
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
6.1
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
10.29
-
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
6.3.5.6
11.8
-
Gln
37C, pH 7.2, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
6.3.5.7
0.027
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.052
2.1
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
6.3.5.7
1.3
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
3
6
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
3.49
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
3.61
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
6.1
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
10.29
-
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
6.3.5.7
11.8
-
Gln
37C, pH 7.2, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
6.3.5.6
S128T
mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln
Helicobacter pylori
6.3.5.6
S152A
mutant is glutaminase inactive
Helicobacter pylori
6.3.5.6
S152T
mutant is glutaminase inactive
Helicobacter pylori
6.3.5.7
S128T
mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln
Helicobacter pylori
6.3.5.7
S152A
mutant is glutaminase inactive
Helicobacter pylori
6.3.5.7
S152T
mutant is glutaminase inactive
Helicobacter pylori
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.3.5.6
0.00095
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.00118
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.0207
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.0224
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.0402
-
Gln
37C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
6.3.5.6
0.0509
-
Gln
37C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity
Helicobacter pylori
6.3.5.6
0.2068
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.00095
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.00118
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.0207
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.0224
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.0402
-
Gln
37C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
6.3.5.7
0.0509
-
Gln
37C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity
Helicobacter pylori
6.3.5.7
0.2068
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.5.6
-
Helicobacter pylori
6.3.5.7
-
Helicobacter pylori
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.3.5.6
ATP + Asp-tRNAAsn + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency
674787
Helicobacter pylori
ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
-
-
?
6.3.5.6
ATP + Glu-tRNAGln + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine
674787
Helicobacter pylori
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
6.3.5.7
ATP + Asp-tRNAAsn + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency
674787
Helicobacter pylori
ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine
674787
Helicobacter pylori
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.3.5.6
0.027
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
0.052
2.1
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
6.3.5.6
1.3
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
3
6
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
3.49
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
3.61
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
6.1
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.6
10.29
-
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
6.3.5.6
11.8
-
Gln
37C, pH 7.2, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori
6.3.5.7
0.027
-
Asn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
0.052
2.1
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
6.3.5.7
1.3
-
Asp-tRNAAsn
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
3
6
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
3.49
-
Gln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
3.61
-
Asp-tRNAGln
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
6.1
-
ATP
37C, pH 7.2, amidotransferase activity
Helicobacter pylori
6.3.5.7
10.29
-
Gln
37C, pH 7.2, cosubstrate: Glu-tRNAGln
Helicobacter pylori
6.3.5.7
11.8
-
Gln
37C, pH 7.2, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity
Helicobacter pylori