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Literature summary extracted from

  • Sheppard, K.; Akochy, P.M.; Salazar, J.C.; Soell, D.
    The Helicobacter pylori amidotransferase GatCAB is equally efficient in glutamine-dependent transamidation of Asp-tRNAAsn and Glu-tRNAGln (2007), J. Biol. Chem., 282, 11866-11873.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.6 S128T mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln Helicobacter pylori
6.3.5.6 S152A mutant is glutaminase inactive Helicobacter pylori
6.3.5.6 S152T mutant is glutaminase inactive Helicobacter pylori
6.3.5.7 S128T mutant protein retains significant glutaminase activity and transamidase activity in the presence of Gln Helicobacter pylori
6.3.5.7 S152A mutant is glutaminase inactive Helicobacter pylori
6.3.5.7 S152T mutant is glutaminase inactive Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.6 0.00095
-
 Asp-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 0.00118
-
 Asp-tRNAGln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 0.0207
-
 Gln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 0.0224
-
 L-aspartyl-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 0.0402
-
 Gln 37°C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity Helicobacter pylori
6.3.5.6 0.0509
-
 Gln 37°C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity Helicobacter pylori
6.3.5.6 0.2068
-
 ATP 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 0.00095
-
 Asp-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 0.00118
-
 Asp-tRNAGln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 0.0207
-
 Gln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 0.0224
-
 L-aspartyl-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 0.0402
-
 Gln 37°C, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity Helicobacter pylori
6.3.5.7 0.0509
-
 Gln 37°C, cosubstrate Glu-tRNAGln + ATP, glutaminase activity Helicobacter pylori
6.3.5.7 0.2068
-
 ATP 37°C, pH 7.2, amidotransferase activity Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.6 Helicobacter pylori
-
-
-
6.3.5.7 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.6
-
 Helicobacter pylori
6.3.5.7
-
 Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.6 ATP + Asp-tRNAAsn + L-glutamine the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency Helicobacter pylori ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
 ?
6.3.5.6 ATP + Glu-tRNAGln + L-glutamine the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine Helicobacter pylori ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?
6.3.5.6 ATP + L-aspartyl-tRNAAsn + L-glutamine + H2O
-
 Helicobacter pylori ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
 ?
6.3.5.7 ATP + Asp-tRNAAsn + L-glutamine the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency Helicobacter pylori ADP + phosphate + Asn-tRNAAsn + L-glutamate
-
 ?
6.3.5.7 ATP + Glu-tRNAGln + L-glutamine the enzyme transamidates Asp-tRNAAsn and Glu-tRNAGln with similar efficiency. GatCAB uses the amide donor glutamine 129fold more efficiently than asparagine Helicobacter pylori ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?
6.3.5.7 ATP + L-aspartyl-tRNAAsn + L-glutamine + H2O
-
 Helicobacter pylori ADP + phosphate + L-asparaginyl-tRNAAsn + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.6 GatCAB
-
 Helicobacter pylori
6.3.5.7 GatCAB
-
 Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.5.6 0.027
-
 L-aspartyl-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 0.052 2.1 Gln 37°C, pH 7.2, cosubstrate: Glu-tRNAGln Helicobacter pylori
6.3.5.6 1.3
-
 Asp-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 3 6 Gln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 3.49
-
 Gln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 3.61
-
 Asp-tRNAGln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 6.1
-
 ATP 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.6 10.29
-
 Gln 37°C, pH 7.2, cosubstrate: Glu-tRNAGln Helicobacter pylori
6.3.5.6 11.8
-
 Gln 37°C, pH 7.2, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity Helicobacter pylori
6.3.5.7 0.027
-
 L-aspartyl-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 0.052 2.1 Gln 37°C, pH 7.2, cosubstrate: Glu-tRNAGln Helicobacter pylori
6.3.5.7 1.3
-
 Asp-tRNAAsn 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 3 6 Gln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 3.49
-
 Gln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 3.61
-
 Asp-tRNAGln 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 6.1
-
 ATP 37°C, pH 7.2, amidotransferase activity Helicobacter pylori
6.3.5.7 10.29
-
 Gln 37°C, pH 7.2, cosubstrate: Glu-tRNAGln Helicobacter pylori
6.3.5.7 11.8
-
 Gln 37°C, pH 7.2, cosubstrate Asp-tRNAAsn + ATP, glutaminase activity Helicobacter pylori