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Literature summary extracted from

  • Storozhenko, S.; Navarrete, O.; Ravanel, S.; De Brouwer, V.; Chaerle, P.; Zhang, G.F.; Bastien, O.; Lambert, W.; Rebeille, F.; Van Der Straeten, D.
    Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response (2007), J. Biol. Chem., 282, 10749-10761.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.3 additional information expression of cytHPPK/DHPS mRNA is induced by salt stress Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.15 expression in Escherichia coli as N-terminal His6 fusion Arabidopsis thaliana
2.5.1.15 expression in Saccharomyces cerevisiae Fol1 knockout Y26466 Arabidopsis thaliana
2.7.6.3 expression in Escherichia coli and Saccharomyces cerevisiae Arabidopsis thaliana
2.7.6.3 isozyme cytHPPK/DHPS DNA and amino acid sequence determination and analysis, and phylogenetic analysis, transient expression of GFP-tagged isozyme cytHPPK/DHPS in the cytosol of Arabidopsis thaliana protoplasts using transfection via Agrobacterium tumefaciens strain GV3101/pMP90, functional complementation of a Saccharomyces cerevisiae mutant, that lacks the enzyme activity, by expression of isozyme cytHPPK/DHPS, expression and subcloning of N-terminally His6-tagged isozyme cytHPPK/DHPS in Escherichia coli strains DH-5alpha and BL-21AI, co-expression with GroESL chaperones Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.15 additional information bifunctional enzyme, mitochondrial enzyme ubiquitously expressed, cytosolic enzyme only in reproductive tisse, construction of a chimeric protein containing the first 224 amino acids of cytosolic DHPS fused to GFP, expression in Arabidopsis thaliana, Arabidopsis thaliana mutant lacking DHPS indicates a role of DHPS in seed stress Arabidopsis thaliana
2.7.6.3 additional information construction of a T-DNA insertion mutant in the cytHPPK/DHPS gene, resulting in lower germination rates as compared with the wild-type upon application of oxidative and osmotic stress, phenotype, overview Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.15 cytosol
-
 Arabidopsis thaliana 5829
-
2.5.1.15 mitochondrion
-
 Arabidopsis thaliana 5739
-
2.7.6.3 chloroplast
-
 Arabidopsis thaliana 9507
-
2.7.6.3 cytosol isozyme cytHPPK/DHPS lacking a potential transit peptide Arabidopsis thaliana 5829
-
2.7.6.3 mitochondrion
-
 Arabidopsis thaliana 5739
-
2.7.6.3 mitochondrion mitHPPK/DHPS Arabidopsis thaliana 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.3 Mg2+
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.3 54053
-
 x * 54053, sequence calculation Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Arabidopsis thaliana
-
 diphosphate + 7,8-dihydropteroate
-
 ?
2.7.6.3 6-hydroxymethyl-7,8-dihydropterin + ATP Arabidopsis thaliana tetrahydrofolate biosynthesis 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
-
 ?
2.7.6.3 ATP + 6-hydroxymethyl-7,8-dihydropterin Arabidopsis thaliana 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase, i.e. mitHPPK/DHPS, is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis, folate biosynthesis in Arabidopsis thaliana, overview AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Arabidopsis thaliana Q1ENB6
-
-
2.7.6.3 Arabidopsis thaliana
-
-
-
2.7.6.3 Arabidopsis thaliana Q1ENB6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 overexpression of the N-terminal His6 fusion protein in Escherichia coli, coexpression with chaperone genes to increase solubility Arabidopsis thaliana
2.7.6.3 recombinant N-terminally His6-tagged isozyme cytHPPK/DHPS from Escherichia coli strains DH-5alpha and BL-21AI Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.3 additional information ubiquitous expression of isozyme mitHPPK/DHPS Arabidopsis thaliana
-
2.7.6.3 seed developing, isozyme cytHPPK/DHPS gene is exclusively expressed in developing seeds, histochemical analysis of a transgenic cytHPPK/DHPS promoter-GUS line Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
 Arabidopsis thaliana diphosphate + 7,8-dihydropteroate
-
 ?
2.7.6.3 6-hydroxymethyl-7,8-dihydropterin + ATP tetrahydrofolate biosynthesis Arabidopsis thaliana 6-hydroxymethyl-7,8-dihydropterin diphosphate + AMP
-
 ?
2.7.6.3 ATP + 6-hydroxymethyl-7,8-dihydropterin
-
 Arabidopsis thaliana AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
 ?
2.7.6.3 ATP + 6-hydroxymethyl-7,8-dihydropterin 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase, i.e. mitHPPK/DHPS, is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis, folate biosynthesis in Arabidopsis thaliana, overview Arabidopsis thaliana AMP + (7,8-dihydropterin-6-yl)methyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.3 ? x * 54053, sequence calculation Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.5.1.15 7,8-dihydropteroate synthase
-
 Arabidopsis thaliana
2.5.1.15 DHPS
-
 Arabidopsis thaliana
2.5.1.15 DHPS/DHPR
-
 Arabidopsis thaliana
2.5.1.15 dihydropteroate synthase
-
 Arabidopsis thaliana
2.7.6.3 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK)
-
 Arabidopsis thaliana
2.7.6.3 cytHPPK/DHPS
-
 Arabidopsis thaliana
2.7.6.3 hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase
-
 Arabidopsis thaliana
2.7.6.3 hydroxymethyldihydropterin pyrophosphokinase (HPPK)
-
 Arabidopsis thaliana
2.7.6.3 mitHPPK/DHPS
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.3 30
-
 assay at Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.3 8
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.6.3 ATP
-
 Arabidopsis thaliana