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Literature summary extracted from

  • Zhao, K.H.; Su, P.; Li, J.; Tu, J.M.; Zhou, M.; Bubenzer, C.; Scheer, H.
    Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120 (2006), J. Biol. Chem., 281, 8573-8581.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.7.5 additional information phycocyanobilin-CpcB(C155I) and phycocyanobilin-PecB(C155I) are also biosynthesized heterologously in vivo, when cpeS is introduced into Escherichia coli with cpcB(C155I) or pecB(C155I), respectively, together with genes ho1 and pcyA Anabaena sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.5 coexpression in Escherichia coli BL21(DE3) with cpcB(C155I) or pecB(C155I) and with cpeS, ho1 and pcyA, resulting in biosynthesis of the respective beta-subunits singly chromophorylated at cysteine-beta84 Anabaena sp.
4.4.1.29 overexpressed in Escherichia coli Anabaena sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.29 Co2+ 5 mM, complete inhibition Anabaena sp.
4.4.1.29 Cu2+ 5 mM, complete inhibition Anabaena sp.
4.4.1.29 Fe2+ 5 mM, 46% inhibition Anabaena sp.
4.4.1.29 Mn2+ 5 mM, 12% inhibition Anabaena sp.
4.4.1.29 additional information no effect up to 20 mM EDTA Anabaena sp.
4.4.1.29 Ni2+ 5 mM, complete inhibition Anabaena sp.
4.4.1.29 Zn2+ 5 mM, complete inhibition Anabaena sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.29 0.0023
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo-[phycoerythrocyanin beta-subunit] mutant C155I Anabaena sp.
4.4.1.29 0.0027
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo-[C-phycocyanin beta-subunit] mutant C155I Anabaena sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.29 NaCl optimal concentration: 150 mM Anabaena sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.29 apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp. the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp. PCC 7120 the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp. the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin Anabaena sp. PCC 7120 the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.5 Anabaena sp.
-
-
-
1.3.7.5 Anabaena sp. PCC 7120
-
-
-
4.4.1.29 Anabaena sp. Q8YZ70
-
-
4.4.1.29 Anabaena sp. PCC 7120 Q8YZ70
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.29
-
 Anabaena sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
4.4.1.29 [allophycocyanin alpha-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin alpha-subunit] + (2R,3E)-phycocyanobilin (3) Anabaena sp.
a
4.4.1.29 [allophycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[allophycocyanin beta-subunit] + (2R,3E)-phycocyanobilin (4) Anabaena sp.
a
4.4.1.29 [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin (1) Anabaena sp.
a
4.4.1.29 [C-phycoerythrin alpha-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin alpha-subunit] + (2R,3E)-phycoerythrobilin (5) Anabaena sp.
a
4.4.1.29 [C-phycoerythrin beta-subunit]-Cys84-phycoerythrobilin = apo-[C-phycoerythrin beta-subunit] + (2R,3E)-phycoerythrobilin (6) Anabaena sp.
a
4.4.1.29 [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin = apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin (2) Anabaena sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.5 biliverdin IXa + reduced ferredoxin
-
 Anabaena sp. (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
1.3.7.5 biliverdin IXa + reduced ferredoxin
-
 Anabaena sp. PCC 7120 (3Z)-phycocyanobilin + oxidized ferredoxin
-
 ?
4.4.1.29 apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins Anabaena sp. [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches (2R,3E)-phycocyanobilin to native apo C-phycocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant Anabaena sp. [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins Anabaena sp. PCC 7120 [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[C-phycocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches (2R,3E)-phycocyanobilin to native apo C-phycocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant Anabaena sp. PCC 7120 [C-phycocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins Anabaena sp. [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches (2R,3E)-phycocyanobilin to native apo phycoerythrocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant Anabaena sp. [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches phycobilin chromophores to cysteine 84 of several phycobiliproteins Anabaena sp. PCC 7120 [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?
4.4.1.29 apo-[phycoerythrocyanin beta-subunit] + (2R,3E)-phycocyanobilin the enzyme attaches (2R,3E)-phycocyanobilin to native apo phycoerythrocyanin beta-subunit and to the C155I-mutant, but not to theC84S mutant Anabaena sp. PCC 7120 [phycoerythrocyanin beta-subunit]-Cys84-phycocyanobilin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.7.5 PcyA
-
 Anabaena sp.
4.4.1.29 alr0617
-
 Anabaena sp.
4.4.1.29 cpeS
-
 Anabaena sp.
4.4.1.29 phycocyanobilin:Cys-beta84-phycobiliprotein lyase
-
 Anabaena sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.29 40
-
 substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 Anabaena sp.
4.4.1.29 45
-
 substrate: apo-[C-phycocyanin beta-subunit] Anabaena sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.4.1.29 30 55 30°C: about 55% of maximal activity, 55°C: about 70% of maximal activity, substrate: apo-[C-phycocyanin beta-subunit] Anabaena sp.
4.4.1.29 30 55 30°C: about 75% of maximal activity, 55°C: about 50% of maximal activity, substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 Anabaena sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.29 0.000011
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo phycoerythrocyanin beta-subunit mutant C155I Anabaena sp.
4.4.1.29 0.000017
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo C-phycocyanin beta-subunit mutant C155I Anabaena sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.29 7 9 substrate: apo-[C-phycocyanin beta-subunit] Anabaena sp.
4.4.1.29 7.5 8 substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 Anabaena sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.29 6.5 9.5 pH 6.5: about 50% of maximal activity, pH 9.5: about 75% of maximal activity, substrate: apo-[C-phycocyanin beta-subunit] Anabaena sp.
4.4.1.29 7 8.5 pH 7.0: about 80% of maximal activity, pH 7.0: about 80% of maximal activity, substrate: apo-[phycoerythrocyanin beta-subunit] mutant C155 Anabaena sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.29 0.0048
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo-[phycoerythrocyanin beta-subunit] mutant C155I Anabaena sp.
4.4.1.29 0.0063
-
 (2R,3E)-phycocyanobilin pH 7.5, 37°C, cosubstrate: apo-[C-phycocyanin beta-subunit] mutant C155I Anabaena sp.