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Literature summary extracted from

  • Chen, Y.M.; Ferrar, T.S.; Lohmeir-Vogel, E.; Morrice, N.; Mizuno, Y.; Berenger, B.; Ng, K.K.; Muench, D.G.; Moorhead, G.B.
    The PII signal transduction protein of Arabidopsis thaliana forms an arginine-regulated complex with plastid N-acetyl glutamate kinase (2006), J. Biol. Chem., 281, 5726-5733.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.8 2-Ketoglutarate only if bound to the PII protein ATP complex Arabidopsis thaliana
2.7.2.8 PII protein prevents inhibition by arginine Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.8 expression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.8 L-arginine less inhibitory in the presence of the PII protein Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.8 1.74
-
 ATP without PII protein Arabidopsis thaliana
2.7.2.8 2.03
-
 ATP in complex with PII protein Arabidopsis thaliana
2.7.2.8 2.47
-
 ATP in complex with PII protein and 2-ketoglutarate Arabidopsis thaliana
2.7.2.8 7.08
-
 N-acetyl-L-glutamate without PII protein Arabidopsis thaliana
2.7.2.8 7.55
-
 N-acetyl-L-glutamate in complex with PII protein Arabidopsis thaliana
2.7.2.8 9.45
-
 N-acetyl-L-glutamate in complex with PII protein and 2-ketoglutarate Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.2.8 chloroplast
-
 Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.8 33000
-
 mass spectrometry Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.8 Arabidopsis thaliana Q9SCL7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.8 affinity chromatography and gel filtration by binding to the PII protein, Ni-nitrilotriacetic acid agarose to purify the recombinant protein Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Arabidopsis thaliana ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.2.8 acetylglutamate kinase
-
 Arabidopsis thaliana
2.7.2.8 N-acetylglutamate kinase
-
 Arabidopsis thaliana
2.7.2.8 NagK
-
 Arabidopsis thaliana
2.7.2.8 NAGS-K
-
 Arabidopsis thaliana