EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.20 | gene DGAT2, expression of FLAG-tagged wild-type and mutant enzyme in COS-7 cells | Mus musculus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.20 | C87S | site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme | Mus musculus |
2.3.1.20 | H161A | site-directed mutagenesis, the mutant shows about 50% reduced activity compared to the wild-type enzyme | Mus musculus |
2.3.1.20 | H161A/P162G/H163A | site-directed mutagenesis, the mutant shows over 80% reduced activity compared to the wild-type enzyme | Mus musculus |
2.3.1.20 | H163A | site-directed mutagenesis, the mutant shows over 80% reduced activity compared to the wild-type enzyme | Mus musculus |
2.3.1.20 | P162G | site-directed mutagenesis, the mutant shows about 40% reduced activity compared to the wild-type enzyme | Mus musculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.20 | membrane | DGAT2 is an integral membrane protein with both the N and C terminus oriented toward the cytosol, the loop between the two transmembrane domains of DGAT2is not exposed to the cytosol | Mus musculus | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.20 | sn-1,2-diacylglycerol + acyl-CoA | Mus musculus | the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2 | triacylglycerol + CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.20 | Mus musculus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.20 | acyl-CoA + 1,2-diacyl-sn-glycerol = CoA + triacylglycerol | the conserved sequence 161HPHG164 is important for activity | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.20 | sn-1,2-diacylglycerol + acyl-CoA | the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2 | Mus musculus | triacylglycerol + CoA | - |
? | |
2.3.1.20 | sn-1,2-diacylglycerol + acyl-CoA | the enzyme contains a neutral lipid binding sequence 80FLVLGVAC87 residing in the first transmembrane domain | Mus musculus | triacylglycerol + CoA | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.20 | acyl-CoA:diacylglycerol acyltransferase-2 | - |
Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.20 | 7.4 | - |
assay at | Mus musculus |