EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.85 | expression of TcSPPS is able to complement an Escherichia coli ispB mutant | Trypanosoma cruzi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.85 | 0.00459 | - |
farnesyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
2.5.1.85 | 0.00707 | - |
geranylgeranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
2.5.1.85 | 0.0192 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranyl diphosphate | Trypanosoma cruzi | |
2.5.1.85 | 0.0243 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate | Trypanosoma cruzi | |
2.5.1.85 | 0.0307 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: farnesyl diphosphate | Trypanosoma cruzi | |
2.5.1.85 | 0.0548 | - |
geranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.5.1.85 | glycosome | - |
Trypanosoma cruzi | 20015 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.85 | Mg2+ | optimal level of activity obtained at 0.5-1 mM. No activation by Mn2+ | Trypanosoma cruzi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.85 | 39000 | - |
x * 39000, calculated from sequence | Trypanosoma cruzi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.85 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | Trypanosoma cruzi | the enzyme is involved in synthesis of ubiquinone-9 | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.85 | Trypanosoma cruzi | Q964Q8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.85 | - |
Trypanosoma cruzi |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.85 | epimastigote | - |
Trypanosoma cruzi | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.85 | (2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate | - |
Trypanosoma cruzi | 6 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.85 | dimethylallyl diphosphate + 8 isopentenyl diphosphate | low activity | Trypanosoma cruzi | 8 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.85 | geranyl diphosphate + 7 isopentenyl diphosphate | - |
Trypanosoma cruzi | 7 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.85 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | geranylgeranyl diphosphate is the preferred allylic substrate | Trypanosoma cruzi | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? | |
2.5.1.85 | geranylgeranyl diphosphate + 5 isopentenyl diphosphate | the enzyme is involved in synthesis of ubiquinone-9 | Trypanosoma cruzi | 5 diphosphate + all-trans-nonaprenyl diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.85 | ? | x * 39000, calculated from sequence | Trypanosoma cruzi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.85 | solanesyl-diphosphate synthase | - |
Trypanosoma cruzi |
2.5.1.85 | SPPS | - |
Trypanosoma cruzi |
2.5.1.85 | TCSPPS | - |
Trypanosoma cruzi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.85 | 0.032 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
2.5.1.85 | 0.043 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: farnesyl diphosphate | Trypanosoma cruzi | |
2.5.1.85 | 0.065 | - |
geranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
2.5.1.85 | 0.087 | - |
geranylgeranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
2.5.1.85 | 0.131 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranyl diphosphate | Trypanosoma cruzi | |
2.5.1.85 | 0.217 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate | Trypanosoma cruzi |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.5.1.85 | Trypanosoma cruzi | calculated from sequence | - |
6.01 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.85 | 0.00119 | - |
geranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
2.5.1.85 | 0.0014 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: farnesyl diphosphate | Trypanosoma cruzi | |
2.5.1.85 | 0.00685 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranyl diphosphate | Trypanosoma cruzi | |
2.5.1.85 | 0.00701 | - |
(2E,6E)-farnesyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi | |
2.5.1.85 | 0.00893 | - |
isopentenyl diphosphate | pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate | Trypanosoma cruzi | |
2.5.1.85 | 0.012 | - |
geranylgeranyl diphosphate | pH 7.4, 37°C | Trypanosoma cruzi |