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Literature summary extracted from

  • Geslain, R.; Aeby, E.; Guitart, T.; Jones, T.E.; Castro de Moura, M.; Charriere, F.; Schneider, A.; Ribas de Pouplana, L.
    Trypanosoma seryl-tRNA synthetase is a metazoan-like enzyme with high affinity for tRNASec (2006), J. Biol. Chem., 281, 38217-38225.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.11 gene encoding SerRS, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression of His-tagged SerRS in Escherichia coli Trypanosoma cruzi
6.1.1.11 gene SerS, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis Trypanosoma brucei

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.11 additional information RNAi-mediated ablation of SerRS using stem-loop constructs containing the puromycin resistance gene, overview Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.11 additional information Trypanosoma brucei is highly sensitive to the action of specific selenoprotein inhibitors Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.11 additional information
-
additional information kinetics Trypanosoma brucei
6.1.1.11 0.0004
-
tRNASecUCA pH 7.6, 37°C, recombinant enzyme Trypanosoma brucei
6.1.1.11 0.0032
-
tRNASerCGA pH 7.6, 37°C, recombinant enzyme Trypanosoma brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.11 Mg2+
-
Trypanosoma brucei
6.1.1.11 Mg2+
-
Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASec Trypanosoma brucei SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins, SerRS is essential for growth Trypanosoma brucei and is responsible for the serylation of both tRNASer and tRNASec AMP + diphosphate + L-seryl-tRNASec
-
?
6.1.1.11 ATP + L-serine + tRNASec Trypanosoma cruzi SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins, SerRS is responsible for the serylation of both tRNASer and tRNASec AMP + diphosphate + L-seryl-tRNASec
-
?
6.1.1.11 ATP + L-serine + tRNASer Trypanosoma cruzi responsible for the serylation of both tRNASer and tRNASec AMP + diphosphate + L-seryl-tRNASer
-
?
6.1.1.11 ATP + L-serine + tRNASer Trypanosoma brucei tRNASec, unlike most trypanosomal tRNAs, is exclusively localized in the cytosol, SerRS is essential for growth Trypanosoma brucei and is responsible for the serylation of both tRNASer and tRNASec AMP + diphosphate + L-seryl-tRNASer
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.11 Trypanosoma brucei
-
gene SerS
-
6.1.1.11 Trypanosoma cruzi Q4CW46 fragment; gene SerS
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.11 recombinant His-tagged SerRS from Escherichia coli by nickel affinity chromatography Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.11 ATP + L-serine + tRNASec SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins, SerRS is essential for growth Trypanosoma brucei and is responsible for the serylation of both tRNASer and tRNASec Trypanosoma brucei AMP + diphosphate + L-seryl-tRNASec
-
?
6.1.1.11 ATP + L-serine + tRNASec SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins, SerRS is responsible for the serylation of both tRNASer and tRNASec Trypanosoma cruzi AMP + diphosphate + L-seryl-tRNASec
-
?
6.1.1.11 ATP + L-serine + tRNASec SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins Trypanosoma cruzi AMP + diphosphate + L-seryl-tRNASec
-
?
6.1.1.11 ATP + L-serine + tRNASecUCA SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins Trypanosoma brucei AMP + diphosphate + L-seryl-tRNASecUCA
-
?
6.1.1.11 ATP + L-serine + tRNASer
-
Trypanosoma cruzi AMP + diphosphate + L-seryl-tRNASer
-
?
6.1.1.11 ATP + L-serine + tRNASer responsible for the serylation of both tRNASer and tRNASec Trypanosoma cruzi AMP + diphosphate + L-seryl-tRNASer
-
?
6.1.1.11 ATP + L-serine + tRNASer tRNASec, unlike most trypanosomal tRNAs, is exclusively localized in the cytosol, SerRS is essential for growth Trypanosoma brucei and is responsible for the serylation of both tRNASer and tRNASec Trypanosoma brucei AMP + diphosphate + L-seryl-tRNASer
-
?
6.1.1.11 ATP + L-serine + tRNASerCGA
-
Trypanosoma brucei AMP + diphosphate + L-seryl-tRNASerCGA
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.11 SerRS
-
Trypanosoma brucei
6.1.1.11 SerRS
-
Trypanosoma cruzi
6.1.1.11 Seryl-tRNA synthetase
-
Trypanosoma brucei
6.1.1.11 Seryl-tRNA synthetase
-
Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.11 37
-
assay at Trypanosoma brucei
6.1.1.11 37
-
assay at Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.11 7.6
-
assay at Trypanosoma brucei
6.1.1.11 7.6
-
assay at Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.11 ATP
-
Trypanosoma brucei
6.1.1.11 ATP
-
Trypanosoma cruzi