EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.20 | - |
Leishmania major |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.20 | additional information | enzyme null mutants lack 5-methyltetrahydrofolate and cannot utilize exogenous homocysteine for growth. In mouse model of infection, null mutants show good infectivity and virulence | Leishmania major |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | additional information | not inhibitory: S-adenosyl-L-methionine, no substrate inhibition up to 0.25 mM | Leishmania major |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.20 | 34800 | - |
x * 34800, calculated | Leishmania major |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.20 | Leishmania major | - |
expression in Saccharomyces cerevisiae | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.5.1.20 | additional information | protein sequence shows no obvious intracellular targeting sequences | Leishmania major |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.20 | 5-methyltetrahydrofolate + NAD+ | - |
Leishmania major | 5,10-methylenetetrahydrofolate + NADH | - |
? | |
1.5.1.20 | 5-methyltetrahydrofolate + NADP+ | - |
Leishmania major | 5,10-methylenetetrahydrofolate + NADPH | - |
? | |
1.5.1.20 | 5-methyltetrahydrofolate + oxidized menadione | - |
Leishmania major | 5,10-methylenetetrahydrofolate + reduced menadione | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.20 | ? | x * 34800, calculated | Leishmania major |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.20 | 52 | - |
NADH | phosphate buffer | Leishmania major | |
1.5.1.20 | 74 | - |
NADPH | phosphate buffer | Leishmania major | |
1.5.1.20 | 87 | - |
NADH | Tris buffer | Leishmania major | |
1.5.1.20 | 140 | - |
NADPH | Tris buffer | Leishmania major |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.20 | NADH | dual-cofactor specificity with NADH and NADPH under physiological conditions | Leishmania major | |
1.5.1.20 | NADPH | dual-cofactor specificity with NADH and NADPH under physiological conditions | Leishmania major |