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Literature summary extracted from

  • Vickers, T.J.; Orsomando, G.; de la Garza, R.D.; Scott, D.A.; Kang, S.O.; Hanson, A.D.; Beverley, S.M.
    Biochemical and genetic analysis of methylenetetrahydrofolate reductase in Leishmania metabolism and virulence (2006), J. Biol. Chem., 281, 38150-38158.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.20
-
Leishmania major

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.20 additional information enzyme null mutants lack 5-methyltetrahydrofolate and cannot utilize exogenous homocysteine for growth. In mouse model of infection, null mutants show good infectivity and virulence Leishmania major

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.20 additional information not inhibitory: S-adenosyl-L-methionine, no substrate inhibition up to 0.25 mM Leishmania major

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.20 34800
-
x * 34800, calculated Leishmania major

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.20 Leishmania major
-
expression in Saccharomyces cerevisiae
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.5.1.20 additional information protein sequence shows no obvious intracellular targeting sequences Leishmania major

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.20 5-methyltetrahydrofolate + NAD+
-
Leishmania major 5,10-methylenetetrahydrofolate + NADH
-
?
1.5.1.20 5-methyltetrahydrofolate + NADP+
-
Leishmania major 5,10-methylenetetrahydrofolate + NADPH
-
?
1.5.1.20 5-methyltetrahydrofolate + oxidized menadione
-
Leishmania major 5,10-methylenetetrahydrofolate + reduced menadione
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.20 ? x * 34800, calculated Leishmania major

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.20 52
-
NADH phosphate buffer Leishmania major
1.5.1.20 74
-
NADPH phosphate buffer Leishmania major
1.5.1.20 87
-
NADH Tris buffer Leishmania major
1.5.1.20 140
-
NADPH Tris buffer Leishmania major

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.20 NADH dual-cofactor specificity with NADH and NADPH under physiological conditions Leishmania major
1.5.1.20 NADPH dual-cofactor specificity with NADH and NADPH under physiological conditions Leishmania major