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Literature summary extracted from

  • Jonczyk, R.; Genschel, U.
    Molecular adaptation and allostery in plant pantothenate synthetases (2006), J. Biol. Chem., 281, 37435-37446.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.1 expression in Escherichia coli as N-terminal His-tagged protein Arabidopsis thaliana
6.3.2.1 expression in Escherichia coli BL21 as N-terminal His-tagged protein Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.1 E132A kcat for beta-alanine is 3.7fold higher than wild-type value, kcat for pantoate is 21fold higher than wild-type value Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.1 (R)-pantoate substrate inhibition Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine Escherichia coli synthesis of pantothenate, the essential precursor to coenzyme A AMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 ATP + (R)-pantoate + beta-alanine Arabidopsis thaliana synthesis of pantothenate, the essential precursor to coenzyme A AMP + diphosphate + (R)-pantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.1 Arabidopsis thaliana
-
-
-
6.3.2.1 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.1
-
 Escherichia coli
6.3.2.1
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.1 ATP + (R)-pantoate + beta-alanine synthesis of pantothenate, the essential precursor to coenzyme A Escherichia coli AMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 ATP + (R)-pantoate + beta-alanine synthesis of pantothenate, the essential precursor to coenzyme A Arabidopsis thaliana AMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 ATP + (R)-pantoate + beta-alanine Bi Uni Uni Bi kinetic mechanism. Enzyme displays non-allosteric behavior Escherichia coli AMP + diphosphate + (R)-pantothenate
-
 ?
6.3.2.1 ATP + (R)-pantoate + beta-alanine the two-step reaction mechanism, large allosteric effects Arabidopsis thaliana AMP + diphosphate + (R)-pantothenate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.1 dimer
-
 Escherichia coli
6.3.2.1 dimer
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.2.1 0.039
-
 (R)-pantoate 25°C, pH 8.0, wild-type enzyme Arabidopsis thaliana
6.3.2.1 0.16
-
 beta-Alanine 25°C, pH 8.0, wild-type enzyme Arabidopsis thaliana
6.3.2.1 0.59
-
 beta-Alanine 25°C, pH 8.0, mutant enzyme E132A Arabidopsis thaliana
6.3.2.1 0.81
-
 (R)-pantoate 25°C, pH 8.0, mutant enzyme E132A Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.1 5.5
-
 exchange between beta-alanine and pantothenate in the presence of AMP Escherichia coli
6.3.2.1 5.5
-
 exchange between beta-alanine and pantothenate in the presence of AMP Arabidopsis thaliana
6.3.2.1 9
-
 synthesis of pantothenate Escherichia coli
6.3.2.1 9
-
 synthesis of pantothenate Arabidopsis thaliana