EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.14.154 | medicine | the enzyme is a potential drug target for treatment of Chagas disease | Trypanosoma cruzi |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.154 | gene CYP51, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli | Trypanosoma cruzi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.154 | I105F | sited-directed mutagenesis, exchange of the animal/fungi-like I105 B' helix residue for the F105 found in this position in all plant and the other six CYP51 sequences from Trypanosomatidae dramatically alters the mutant substrate specificity, activity with C4-methylsterol substrate is 3.5fold reduced and activity with norlanosterol and obtusifoliol is increased 150fold and 60fold, respectively, overview | Trypanosoma cruzi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.154 | 14alpha-aminomethyl-lanosterol derivatives | competitive | Trypanosoma cruzi | |
1.14.14.154 | fluconazole | and analogues | Trypanosoma cruzi | |
1.14.14.154 | additional information | inhibition kinetics and mechanism | Trypanosoma cruzi |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.154 | membrane | - |
Trypanosoma cruzi | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.154 | Iron | a heme iron, DELTA78 double bond location is more favorable for coordination of the amino nitrogen to the heme iron | Trypanosoma cruzi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.154 | 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Trypanosoma cruzi | - |
? | - |
? | |
1.14.14.154 | 24-methylenedihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Trypanosoma cruzi | - |
? | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Trypanosoma cruzi | - |
? | - |
? | |
1.14.14.154 | norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 | Trypanosoma cruzi | i.e. 4alpha,4alpha-dimethylcholesta-8,24-dien-3beta-ol | ? | - |
? | |
1.14.14.154 | obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | Trypanosoma cruzi | preferred substrate | 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.154 | Trypanosoma cruzi | Q7Z1V1 | gene CYP51 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.154 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, and anion and cation exchange chromatography | Trypanosoma cruzi |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.14.154 | a 14alpha-methylsteroid + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 = a DELTA14-steroid + formate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | reaction mechanism, phyla-specific residues in CYP51 | Trypanosoma cruzi |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.14.154 | additional information | - |
substrate specificities of recombinant wild-type and mutant I105F enzymes, overview | Trypanosoma cruzi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.154 | 24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Trypanosoma cruzi | ? | - |
? | |
1.14.14.154 | 24-methylenedihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Trypanosoma cruzi | ? | - |
? | |
1.14.14.154 | lanosterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Trypanosoma cruzi | ? | - |
? | |
1.14.14.154 | additional information | substrate specificity, the Trypanosoma cruzi enzyme prefers C4-dimethylsterols substrates, overview | Trypanosoma cruzi | ? | - |
? | |
1.14.14.154 | norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 | i.e. 4alpha,4alpha-dimethylcholesta-8,24-dien-3beta-ol | Trypanosoma cruzi | ? | - |
? | |
1.14.14.154 | obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 | preferred substrate | Trypanosoma cruzi | 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.154 | CYP51 | - |
Trypanosoma cruzi |
1.14.14.154 | sterol 14alpha-demethylase | - |
Trypanosoma cruzi |