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Literature summary extracted from

  • Kunz, R.C.; Horng, Y.C.; Ragsdale, S.W.
    Spectroscopic and kinetic studies of the reaction of bromopropanesulfonate with methyl-coenzyme M reductase (2006), J. Biol. Chem., 281, 34663-34676.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.4.1 1-butanesulfonate
-
Methanothermobacter marburgensis
2.8.4.1 1-propanesulfonate
-
Methanothermobacter marburgensis
2.8.4.1 2-bromoethanesulfonate
-
Methanothermobacter marburgensis
2.8.4.1 3-bromopropanesulfonate irreversible, strong inhibitor and competitive substrate Methanothermobacter marburgensis
2.8.4.1 3-Bromopropionate
-
Methanothermobacter marburgensis
2.8.4.1 3-chloropropanesulfonyl chloride
-
Methanothermobacter marburgensis
2.8.4.1 3-mercapto-1-propanesulfonate
-
Methanothermobacter marburgensis
2.8.4.1 4-bromobutanesulfonate
-
Methanothermobacter marburgensis
2.8.4.1 4-bromobutyrate
-
Methanothermobacter marburgensis
2.8.4.1 4-Chlorobutyrate
-
Methanothermobacter marburgensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.4.1 additional information
-
additional information steady-state and rapid kinetics, MCRred1-catalyzed cleavage of the C-S bond of methyl-SCoM requires the other substrate, HSCoB, even under single turnover conditions Methanothermobacter marburgensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.1 methyl-coenzyme M + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) Methanothermobacter marburgensis i.e. methyl-SCoM methane + CoB-S-S-CoM a the mixed disulfide ?
2.8.4.1 additional information Methanothermobacter marburgensis MCR also appears to initiate anaerobic methane oxidation, the reverse methanogenesis ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothermobacter marburgensis
-
i.e. Methanothermobacter thermoautotrophicum strain Marburg
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.1 purification of native MCRox1, and of native MCRred1, the latter by ammonium sulfate fractionation and ion exchange chromatography Methanothermobacter marburgensis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.4.1 methyl-CoM + CoB = CoM-S-S-CoB + methane two possible catalytic mechanisms, overview Methanothermobacter marburgensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 methyl-coenzyme M + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) i.e. methyl-SCoM Methanothermobacter marburgensis methane + CoB-S-S-CoM a the mixed disulfide ?
2.8.4.1 additional information MCR also appears to initiate anaerobic methane oxidation, the reverse methanogenesis Methanothermobacter marburgensis ?
-
?
2.8.4.1 additional information conversion of MCRox1 toMCRred1 by Ti(III)citrate, bromopropanesulfonate is an alternative substrate of MCR in an ionic reaction that is coenzyme B-independent and leads to debromination of bromopropanesulfonate and formation of a distinct state with an EPR signal that is assigned to a Ni(III)-propylsulfonate species, propanesulfonate formation also occurs in steady-state reactions in the presence of Ti(III) citrate Methanothermobacter marburgensis ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.8.4.1 methyl-coenzyme M reductase
-
Methanothermobacter marburgensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.4.1 20 25 assay at Methanothermobacter marburgensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.8.4.1 4 65
-
Methanothermobacter marburgensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.4.1 additional information
-
pH 10.0 is optimal for activation of MCRox1 to MCRred1 with Ti(III) citrate Methanothermobacter marburgensis
2.8.4.1 7 7.6 assay at Methanothermobacter marburgensis

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 coenzyme F430 the nickel-containing tetrapyrrole is essential for the reaction, and is bound to the active site Methanothermobacter marburgensis