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Literature summary extracted from

  • Mangmool, S.; Haga, T.; Kobayashi, H.; Kim, K.M.; Nakata, H.; Nishida, M.; Kurose, H.
    Clathrin required for phosphorylation and internalization of beta2-adrenergic receptor by G protein-coupled receptor kinase 2 (GRK2) (2006), J. Biol. Chem., 281, 31940-31949.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 cytosolic expression of wild-type and mutant GRK2 in HEK-293 cells, co-expression with the beta-adrenergic receptor Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.15 additional information mutation of the clathrin-binding motif of GRK2 results in a dominant negative form of GRK2 without affecting kinase activity itself and the ability to interact with beta2-adrenergic receptor, no translocation of beta-adrenergic receptors to endocytic vesicles as well as GRK2 translocation to the plasma membrane with the dominant-negative mutant and mutant GRK2-5A, overview Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.15 plasma membrane the wild-type GRK2 is translocated to the plasma membrane Rattus norvegicus 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.15 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor Rattus norvegicus phosphorylation and internalization of the receptor requires clathrin, GRK2 specifically phosphorylates the activated form of the receptor that promotes the translocation of beta-arrestins to the plasma membrane, overview ADP + phosphorylated beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + rhodopsin Rattus norvegicus light-activated rhodopsin ADP + phosphorylated rhodopsin
-
 ?
2.7.11.15 additional information Rattus norvegicus GRK2 activity leads to translocation of parts of beta-adrenergic receptors to endocytic vesicles ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor phosphorylation and internalization of the receptor requires clathrin, GRK2 specifically phosphorylates the activated form of the receptor that promotes the translocation of beta-arrestins to the plasma membrane, overview Rattus norvegicus ADP + phosphorylated beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor urea-treated rod outer segments as substrate Rattus norvegicus ADP + phosphorylated beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + rhodopsin light-activated rhodopsin Rattus norvegicus ADP + phosphorylated rhodopsin
-
 ?
2.7.11.15 additional information GRK2 activity leads to translocation of parts of beta-adrenergic receptors to endocytic vesicles Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.15 G protein-coupled receptor kinase 2
-
 Rattus norvegicus
2.7.11.15 GRK2
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.15 7.5
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Rattus norvegicus