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Literature summary extracted from

  • Nakamura, S.; Oda, M.; Kataoka, S.; Ueda, S.; Uchiyama, S.; Yoshida, T.; Kobayashi, Y.; Ohkubo, T.
    Apo- and holo-structures of 3alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831. Loop-helix transition induced by coenzyme binding (2006), J. Biol. Chem., 281, 31876-31884.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.50 purified enzyme with NADH, hanging drop vapour diffusion method, 4°C, in presence of 1.4 M ammonium sulfate, 0.14 M NaCl, and 0.1 M Tris, pH 9.0, rod cluster crystals appear within 1 week, X-ray diffraction structure determination and analysis at 1.8 A resolution, modeling Pseudomonas sp.
1.1.1.213 purified enzyme with NADH, hanging drop vapour diffusion method, 4°C, in presence of 1.4 M ammonium sulfate, 0.14 M NaCl, and 0.1 M Tris, pH 9.0, rod cluster crystals appear within 1 week, X-ray diffraction structure determination and analysis at 1.8 A resolution, modeling Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 26000
-
 2 * 26000 Pseudomonas sp.
1.1.1.213 26000
-
 2 * 26000 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ Pseudomonas sp.
-
 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 ?
1.1.1.213 5alpha-dihydrotestosterone + NAD(P)H + H+ Pseudomonas sp.
-
 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Pseudomonas sp. Q59718
-
-
1.1.1.213 Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Pseudomonas sp. 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 ?
1.1.1.213 5alpha-dihydrotestosterone + NAD(P)H + H+
-
 Pseudomonas sp. 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.50 dimer 2 * 26000 Pseudomonas sp.
1.1.1.50 More comparison of the folding topologies found in the crystal structures of the holo-enzyme and the apo-enzyme, structural changes in solution, overview Pseudomonas sp.
1.1.1.213 dimer 2 * 26000 Pseudomonas sp.
1.1.1.213 More comparison of the folding topologies found in the crystal structures of the holo-enzyme and the apo-enzyme, structural changes in solution, overview Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.50 3alpha-hydroxysteroid dehydrogenase
-
 Pseudomonas sp.
1.1.1.50 3alphaHSD
-
 Pseudomonas sp.
1.1.1.50 More cf. EC 1.1.1.213, the enzyme belongs to the AKR1C family Pseudomonas sp.
1.1.1.213 3alpha-hydroxysteroid dehydrogenase
-
 Pseudomonas sp.
1.1.1.213 3alphaHSD
-
 Pseudomonas sp.
1.1.1.213 More cf. EC 1.1.1.50, the enzyme belongs to the AKR1C family Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 NAD(P)H cofactor binding structure, overview Pseudomonas sp.
1.1.1.213 NAD(P)H cofactor binding structure, overview Pseudomonas sp.