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Literature summary extracted from

  • Southall, S.M.; Doel, J.J.; Richardson, D.J.; Oubrie, A.
    Soluble aldose sugar dehydrogenase from Escherichia coli: a highly exposed active site conferring broad substrate specificity (2006), J. Biol. Chem., 281, 30650-30659.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 into the pET M-11 vector for transformation of DH5alpha and BL21DE3 cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.5.2 apo- and holoenzyme at a resolution of 1.5 A Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 150
-
maltotriose
-
Escherichia coli
1.1.5.2 170
-
maltose
-
Escherichia coli
1.1.5.2 400
-
D-glucose
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.2 Ca2+
-
Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 40000
-
determined by SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone Escherichia coli
-
D-glucono-1,5-lactone + ubiquinol
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.2 using a Ni-NTA column, the hexahistidine tag is removed by cleavage with tobacco edge virus protease, cleaved protein is purified by gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 D-glucose + ubiquinone
-
Escherichia coli D-glucono-1,5-lactone + ubiquinol
-
?
1.1.5.2 maltose + ubiquinone
-
Escherichia coli ? + ubiquinol
-
?
1.1.5.2 maltotriose + 2,6-dichlorophenolindophenol
-
Escherichia coli ?
-
r

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 aldose sugar dehydrogenase
-
Escherichia coli
1.1.5.2 Asd
-
Escherichia coli
1.1.5.2 quinoprotein glucose dehydrogenase
-
Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.5.2 25
-
activity assay Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.2 2270
-
maltotriose
-
Escherichia coli
1.1.5.2 2870
-
maltose
-
Escherichia coli
1.1.5.2 3360
-
D-glucose
-
Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 7
-
activity assay Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone PQQ Escherichia coli