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Literature summary extracted from

  • Molle, V.; Brown, A.K.; Besra, G.S.; Cozzone, A.J.; Kremer, L.
    The condensing activities of the Mycobacterium tuberculosis type II fatty acid synthase are differentially regulated by phosphorylation (2006), J. Biol. Chem., 281, 30094-30103.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.294 expressed in Escherichia coli BL21(DE3)pLysS and Mycobacterium bovis BCG Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.294 0.01
-
 malonyl-[acyl-carrier protein M] at pH 7.0 and 37°C Mycobacterium tuberculosis
2.3.1.294 0.0119
-
 palmitoyl-[acyl-carrier protein M] at pH 7.0 and 37°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Mycobacterium tuberculosis
-
 an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] Mycobacterium tuberculosis H37Rv
-
 an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.85 Mycobacterium tuberculosis
-
-
-
2.3.1.294 Mycobacterium tuberculosis
-
-
-
2.3.1.294 Mycobacterium tuberculosis H37Rv
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.85 phosphoprotein condensing activities of the Mycobacterium tuberculosis type II fatty acid synthase are differentially regulated by phosphorylation Mycobacterium tuberculosis
2.3.1.294 phosphoprotein phosphorylation increases the activity of the enzyme in the elongation process of long chain fatty acids synthesis Mycobacterium tuberculosis

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.294 glutathione-Sepharose 4B column chromatography and Ni-NTA agarose bead chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.294 an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis H37Rv an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein]
-
 ?
2.3.1.294 palmitoyl-[acyl-carrier protein M] + malonyl-[acyl-carrier protein M]
-
 Mycobacterium tuberculosis ?
-
 ?
2.3.1.294 palmitoyl-[acyl-carrier protein M] + malonyl-[acyl-carrier protein M]
-
 Mycobacterium tuberculosis H37Rv ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.294 ? x * 46400, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.85 FAS-II
-
 Mycobacterium tuberculosis
2.3.1.85 type II fatty acid synthase
-
 Mycobacterium tuberculosis
2.3.1.294 beta-ketoacyl AcpM synthase
-
 Mycobacterium tuberculosis
2.3.1.294 KasB
-
 Mycobacterium tuberculosis